DIY Mushroom Cultivation Guide
DIY Mushroom Cultivation Guide
us | digg |
reddit | Twitter | facebook
PF TEK
Spore Study
INTRODUCTION
The PF TEK is basically a brown rice method with an improved formula by using vermiculite as a base
and adding pulverized brown rice. The secret is in the vermiculite. When mycelium is cultured in just
grain, the mycelium turns into a mass with little air space. But when grown with vermiculite, the
mycelial threads stretch across space. The important thing about the PF TEK, is that it copies nature.
Instead of the usual cloning of mushroom tissue and growing mushrooms from that, a mass spore
inoculation is employed directly to the fruiting substrate. That way, the genotype remains complete.
Senescence (mutating and ceased fruiting) is no longer a problem. The spores insure a never ending
succession of fungus, with all the power of the spores reproductive ability intact.
OVERVIEW OF PF TECHNIQUES
1. Brown rice powder, vermiculite and distilled water are mixed and loaded into a 1/2 pint jar, which is
steam sterilized. The jar is then inoculated by the spore syringe.
2. After the substrate cake in the jar colonizes and begins to show signs of fruiting, the cake is released
from the jar and placed into the dual chambered terrarium to fruit.
PF SUBSTRATE FORMULATION
Jars and glasses to be used with this technique are 1/2 pint capacity (8 ounces) - (250 milliliters). They
must have tapered sides and no shoulders, otherwise the fungus cakes won't easily come out of the jars.
NOTE: Even though the regular mouth BALL half pint and the regular mouth KERR half pint look
similar, the KERR is not tapered.
1/8 cup of brown rice powder (Health food stores and co-ops)
1/2 - 2/3 cup of horticultural vermiculite (medium grade) (garden centers and hardware)
40-45 cc's (milliliters) of water or (a little less than 1/4 cup) (1&1/2 ounces) (3 tablespoons + 1 teaspoon)
Not all vermiculite is the same. The coarseness varies quite considerably among different brands. The
coarser type will hold less water than the finer type which will alter the water holding capacity. If the
formulation (water content) results in a really wet or sloppy substrate, use less water. Keep notes on
formulas for replicating the substrate formula that fruits the best.
The above formulas utilize the finer type of vermiculite. If the above maximum fruiting formula is used
with the finer type of vermiculite, the jar lid should be loose during incubation (see - "The canning jar lid
- loose or tight").
The finer type of vermiculite is recommended over the coarser type because it holds more water. To
ascertain the size of the vermiculite particles, observe them under a photo magnifier next to a millimeter
ruler. The finer type of vermiculite has particles averaging around 1 millimeter across (some larger and
some smaller). The coarser type has particles averaging around 4 or 5 millimeters across and up to 8
millimeters. Stores usually carry one type. Plus, there will be regional differences in the different brands
of vermiculite. Shop around and try to get both types to compare.
To make homemade brown rice powder, place some regular brown rice in a small canister type coffee
bean grinder and grind it to fine powder. Freshly ground brown rice is recommended over prepackaged
type. The freshness sometimes makes a big difference.
If the measuring cup specs aren't true, the formulas will be off, setting up certain failure or diminished
growth. Check the cup measurers this way: 1 cup is 237 milliliters which is 1/2 pint or 8 liquid ounces
(English measurement). There are 2 cups in a pint, 2 pints in a quart and 4 cups in a quart.
When using two piece canning jar lids, the inner lid
(with the rubber edges up) rests on the top of the jar
and when the lid band is screwed off, the lid remains
resting on the jar top. To make the lid and band act
as one lid, place pieces of masking tape on the lid
attaching the band to the lid. Then, the lid can be
adjusted for air ventilation and looseness like an
ordinary one piece jar lid.
A 3 piece vegetable steamer (pot, basket insert & lid) is used for the steam sterilizing stage. Also, the
stainless steel vegetable steamers that fold out and stand on the bottom of the pot are good. Anything is
good as long as it keeps the jar bottoms off the pot bottom where the high temperature will crack the
glass.
Step 1. Place 1/2 cup of vermiculite into a mixing bowl. Place the brown rice powder on top of the
vermiculite. Slowly add the water directly onto the brown rice powder, wetting it first. Thoroughly mix
the ingredients. The mixture should feel damp and cohesive. More water (or less) can be used if
experimenting to improve the fruiting. Mix Each jars substrate individually for loading to insure accurate
formula rendering.
A note on water: A recent update is worth mentioning. Water quality is indeed important. I have found
out that "natural" water is the water to use. It makes for better cultivation of this mushroom on this
simple substrate. Distilled water is good for making spore solutions and syringes and storing spore
solution. But for growing, they seem to like the "natural" water such as: swamp, lake, stream,
pond,river, ground or any water that is rich in organics. I have heard that "mineral" type drinking water
is good and makes a difference. I suppose that water seeping from an organic compost pile would be
about the best.
Step 2. Fill the jar very loosely. Leave a 1/2 to 3/4 inch space at the top. Level the substrate. With a
tissue or a fingertip, wipe the insides of the jar down to the substrate. Fill the top of the jar with plain dry
vermiculite and level it off at the top. This upper layer will protect the wet substrate from air borne
contaminants. It acts as a contaminant barrier. This is a Psylocybe Fanaticus original discovery. What
this dry vermiculite layer does is protect the wet substrate from airborne contaminants and also absorbs
and regulates moisture transpiration and condensation.
In the photo, the black tape is the depth for the dry
vermiculite. The masking tape shows where the pf
substrate goes. The top layer of dry vermiculite must
be between 1/2" to 2/3" deep to provide protection
from contaminants entering from above.
Step 3. Place the lid on the jar with the rubberized edge up (jagged edges of the needle holes down).
Screw the lid band on. Place pieces of "professional" grade masking tape (holds on during steaming)
over the needle holes. This is to protect the needle holes from contaminant entry.
Step 4. Heat the pot of water to a boil. Put the jars into the pot with the lid bands loose so that the steam
can penetrate the jars quickly. The jars can sit in water but make sure boiling water can't slosh into the
jars. Turn the heat down and GENTLY steam the jars at the lowest possible boil for an hour in a
TIGHTLY covered pot (gas stoves are the easiest to control). A good tight fitting pot lid is essential for
successful steaming.
When steaming or pressure canning is performed, the jars must be protected from water dripping down
from the underside of the pot lid caused by heavy condensation and drip off during boiling. This water
can get into the jars by entering under the jar lids that aren’t tight and soaking the substrate - throwing
off the formula and setting up failure. To prevent this, wrap some tin foil around the cap to ward off the
water. The tin foil can be removed after steaming (with the tape guarding the needle holes - or the tin foil
can be left on until it is inoculation time.)
Be careful to not overheat the jars, this dries the substrate. Drying is evidenced by o.k. spore germination
and halted growth. The fungus will spread but stop at a certain point depending on how dry the substrate
has become. Generally, any halted growth (with no contamination) is a sign of dried substrate. This is an
important concept that will enable diagnosis and correction of problems experienced with drying. The
remedy is to increase the water content of the substrate formula in use. After the jars have cooled, tighten
the lids and store them in a cool draft free place until ready to inoculate them.
Sterilization times
Jars can be inoculated without using a lid with holes punched. Before trying this technique, inoculate
with the punched lid first. That will show how it works without any problems (almost fail proof).
The only precaution to observe is to disturb the dry top vermiculite layer as little as possible, especially
when removing the needle after the inoculation. The underlying substrate must not be exposed to the air.
Carefully move any disturbed vermiculite back into place. If using a drinking glass or alternate
container, cover the mouth with tin foil. Replace the tin foil cover after inoculation.
After the substrate turns white with the mycelium (2 or 3 weeks after inoculation), the jars are left to sit
in indirect light. The mycelium will continue to infiltrate the substrate until it gets enough food to trigger
the fruiting cycle. In less than a week to a few weeks after surface colonization of the cake, tiny white
"pin" like structures begin to appear. This is called pinning. This is the beginning of the fruiting cycle.
Soon after that, within the week, small round fungus growths appear that soon begin to turn yellow.
Lastly, "primordia" start to grow. These are tiny worm like structures with tiny reddish heads. These are
the first mushrooms.
The best time to remove the fungus cake from the jar
is when the primordia (tiny worm like structures with
reddish heads) appear on the cake while still in the
jar. Be careful not to damage them in handling. The
rule is to handle with care.
As soon as the fungus cake comes out of the jar, daub the cake with a piece of loose tissue paper to soak
up any water droplets that may have deposited on the cake as it comes out of the jar. Immediately after
the birthday, place the cakes into the dual chambered terrarium for the fruiting cycle.
This is the cake a few days after the birthday. This is a healthy
fruiting start. Some of these primordia will abhort, but most will
go on to full developement.
The secret to potent mushrooms is in their age when picked. It has been scientifically proven that the
small immature specimens are significantly more potent than the larger mature specimens. Over half of
the small primordia that first form will abhort (cease growing, convolute and deform). Pick these before
their heads turn black. A pointed knife blade works well for removing these high potency primodia.
These are among the most potent. The abortive mushrooms are also high potency. Harvest them when
they are young and before their heads turn black. When the fruitbodies are normal, harvest them before
the veil under the cap breaks. The mushrooms will be smaller and their heads will be roundish. It is
important to note that the mushroom cakes pictured in this book are all mostly well matured. While these
mature specimens are beautiful and perfect, they are not as potent as the diminutive specimens. The
mature specimens are good for spore collecting and showcasing but are weak in psychedelic potency.
Grow them on brown rice, harvest them when they are young and cool dry them with desiccant.
When this is done, they are an entheogen of the highest order.
All in all, the process takes from 4 - 6 weeks from spore inoculation to fruiting.
NON-GERMINATION OF SPORES
1. The spore solution was not inoculated deep enough down into the jar. Instead of running down the
side of the jar and inoculating the substrate cake, the solution was absorbed by the non-nutritive top
vermiculite layer. To avoid this from happening, make sure that the spore solution flows down along the
sides of the substrate cake by inserting the syringe needle so that the tip is below the non-nutritive upper
vermiculite layer.
2. The substrate jars were not allowed to cool down after sterilization, killing the spores. Inoculate only
when the jar feels cool to the touch.
3. There is evidence now that syringe boxes can be exposed to killing heat during transit (a very rare
occurrence). The possibilities are such as over heated airplane cargo holds during intense heat waves or
a superheated mail truck parked all day in the sun. Another possibility is that on arriving at the mail box,
the syringe package was allowed to sit inside a broiling sun heated mail box, killing the spores.
4. Spore syringes can survive freezing, but extreme low temperatures are probably destructive to the
spores.
The wooden lid frames' inner rectangular cutout must be LARGER than the top of the aquarium. Clear
polyethylene plastic film is tacked to the underside (or upper side) of the frame so that the frame holds it
tightly onto the aquarium top. The frame essentially hangs by the plastic film. A simpler alternative is to
cover the aquarium top with saran wrap or something similar. The most important point to be stressed is
that the aquarium must be sealable with no air leaks, for humidity retention.
Use 1/8" thick clear acrylic (Plexiglas) window insulation available at most hardware stores. Have it cut
around 15" x 18" (dimensions may vary - check the aquarium first). A loose fit is good as long as the
cakes are protected from the direct spray.
It all starts with the spray from the hand sprayer. The first rule is to never directly spray the fungus. This
initial spray is comprised of water droplets that are giant ponds of water in relation to the fine mycelial
networks of the fungal threads. In culture, the droplet of water will drown the micro world of the fungal
structures and thereby inhibit or contaminate growth. But the airborne molecularized water floats into the
fine structures and gives the fungus humidity as needed. Molecularized water is another way of
describing water that has evaporated into the air.
The spray that comes out of the spray bottle must be molecularized for the fungus. The spray shield and
the primary chamber accomplish this. The primary chamber receives the initial spraying. As the spray
strikes the shield, it is broken down into a finer mist which flows around the sides of the spray shield
into the secondary chamber where the fungus is bathed in the fine humidity safely away from water
droplets. In a matter of time, this humidity will condense out onto surfaces inside the terrarium and drip
down. The spray shield is slanted and therefore acts as a drip shield and roof, so the more condensation
the better.
SPRAYING PROCEDURE
First, before placing the cakes into the terrarium, spray all the inside surfaces of the terrarium, including
the spray shield and lid. Insert the fungus cakes and put the spray shield and lid in place. Then, slightly
lift up the lid and insert the nozzle of the water spray bottle in between the lid and the top of the
aquarium and vigorously spray downwards into the middle of the shield. After about 5 seconds of
spraying, immediately withdraw the sprayer nozzle and let down the lid to seal the swirling mist inside
the terrarium. Come back after a few minutes and give it another spraying if desired and a third if the
terrarium is to be left untended until the evening. To maintain a high humidity try to spray 2 times a day,
and the more the better. Compensate for a lack of spraying during the day by spraying several times in
the evening. Make sure that all the inside surfaces of the terrarium are foggy or dripping with water. This
in itself helps generate humidity.
It has been seen that mushrooms will grow in a properly set up dual chambered terrarium, with
only one good spraying a day - and even less than that!
Each time the terrarium is sprayed, the fungus should be ventilated. To ventilate, take off the lid, and
while holding the spray shield vertically, fan the chamber with a piece of cardboard, and then spray as
above. Also, the water that collects in the bottom of the terrarium must be siphoned out (prevents
bacteria buildup). This can be easily done using a rubber bulb battery filler (auto parts store) or a rubber
bulb type enema bottle.
Expose the terrarium to normal room light (indirect sunlight). A small low wattage fluorescent plant light
will make the phototropic mushrooms grow upwards. Leave it on all the time if desired.
HEATING
The main rule is to not heat the dual chambered terrarium. Any direct heating works against the
humidification and adds a drying influence. Do not use heating cables, heat pads or blankets. Don't
shine light directly down into the terrarium. Keep any plant grow light (low wattage only) a safe
distance from the terrarium. These fungi grow well at 60 degrees Fahrenheit. PF has even seen them
growing perfectly at temperatures cooler than 60 degrees. They grow slowly when they are cool. When
warm or at heated room temperature, they grow very fast. Strive for a growing temperature between 65
and the upper 80's. A too hot terrarium will result in lots of spreading mycelium, but no fruiting.
Deformed, convoluted, and withering mushrooms and primordia are signs of low humidity. For the best
growth, the humidity has to be very high.
It works great with perlite (and without) and holds 9 half pint cakes.
The one above has 7 cakes. The cakes are post initial flush and the
fruitings are secondary fruitings. The fruitbodies that appear late are
always superb in form.
If you can't find one of these in your local town, you can order one for
around $75 (hence - the "richmans'"). Call the manufacturer (Cal-Mil)
in California at 1 800 321 9069. They will tell you where you can
order it (from one of their distributers near your town).
Unfortunately, sometimes a distributer will require a minimum of an order for two. But fortunately, the
terrarium they will send you for the above price is bigger than the one pictured. It has room for several
more cakes, making for a goodly capacity. All the shrooms pictured at this site were grown in one of
these.
To use this terrarium, first spray the insides. Place the cakes in. Hold the cover above the tray and spray
a fine mist into the air about 2 feet above the cakes in the tray and immediately lower the cover down
onto the tray - trapping mist. Air and mist once a day. But what is so cool about this, is that neglect goes
a long way! (set it up and leave it).
Yeah, I know it costs, but it works so nicely, and it makes a nice coffee table display. If your landlord
comes by to inspect, he will see it, look down and say, "hey, nice shrooms, what kind are they"? Then
you say, "I got it from a science catalog company and it is a new miniature fungi growing kit and the
shrooms are not edible - just wild". (or something absurd like that). Then your landlord will look
approvingly around, notice the neatness and tiddyness of your domecile, and leave, little knowing that
he just observed the food of the gods.
MATERIALS NEEDED - Desiccant - Wire screen - Plastic tub or container - Plastic bag with tie off.
DESICCANT SOURCES
1. "DRIERITE" desiccant. (chemical and science supply retailers). It is the universal lab desiccant.
2. Silica Gel granules - desiccant. (Chemical and science supply)
3. "DAMP GONE" - (looks like kitty litter - for drying closets and damp places in the home) - available
at well stocked hardware stores. This is inexpensive desiccant but works as well as any.
Note: These products might have toxicity warnings - (don't breathe the dust and try not to touch it
directly - it dries skin.). Follow those rules, but know that desiccant in an airtight box and under a screen
will do nothing to the fungi except dry them. It is completely safe for this use.
What desiccant does, is absorb moisture out of the air. As the fungus transpires moisture, the moisture is
immediately absorbed back into the desiccant, drying the fungi. Desiccant can be reused and lasts
indefinitely. After use, the desiccant is heated, dried and stored for future use. Store it in an air tight
container so that it stays dry and ready for use. Heat the desiccant in an oven as instructed by the
manufacturer. This preheating should be done before the desiccant is used because when it is purchased
- it is usually somewhat damp which will thwart its function for drying air.
For the best alkaloid preservation technique, the desiccant box can be put into the refrigerator and the
mushrooms dried at near freezing temperatures.
Pre drying the mushrooms in the air on a wire screen works very well if the the room humidity is not
high. After a couple of days, the shriveling fungus can be quickly and completely dried in the desiccant
box.
Mushrooms dried in this way lose hardly any chemical constituents and their truly desiccated state
preserves them in their prime for months.
1. Presterilize the jar and regular metal lid (rubber edge up) in a small toaster oven at around 300 degrees
Fahrenheit for around a half hour. Keep the lid loose during the sterilization cycle. When the jar has
cooled down, tighten the lid until it is time to use the jar for a spore print. The rubberized edge will be a
bit melted, but that won't be any problem in this technique.
Note: What follows is a sterile technique. The first rule that must be always followed is to wash hands
prior to sterile work. Hands are a prime source for bacteria and microspore contaminants. Sterilize all the
work surfaces with rubbing alcohol. Minimize drafts. Try for a still air environment. Don't breathe on
the work. Run a small home appliance style HEPA air cleaner (99.97% rated efficiency - available at
drug and department stores) for a few hours in a closed room to clean the air before doing sterile work.
2. Flame sterilize the scissors with an alcohol flame and snip off the mushroom cap. Cut the top of the
stem as far up into the cap as possible so that the gills of the mushroom will sit flat on the surface of the
jar bottom. With quick and sure movements, place the cap into the jar and place the lid on loosely.
Pierce the top of the cap with a straight pin to pick it up and handle it.
3. Leave the jar with a loose cap for a couple of days in a draft free area away from direct sunlight. After
the print is taken, quickly and with as little air disturbance as possible, remove the jar cap and extract the
mushroom cap from the jar. With a loose jar cap, let the jar sit in a draft free place to dehumidify for a
few days before sealing it up (with tape) because there will be some residual moisture left behind on the
spores and glass. Store the spore print jar at room temperatures in a dark place away from sunlight. Don't
store it in a refrigerator.
Psilocybe Cubensis spores begin to degrade a few months after they are taken. After approximately 1
1/2 years, spore germination will be greatly reduced or won't occur at all. Germination is massive and
quick when the spores are fresh.
Syringe preparation
Boil a pot of water. Draw boiling water into a syringe and squirt it out several times. Refill the syringe
with boiling water, replace the needle quard and wrap the syringe in tin foil. Prepare several syringes
like this. Drop the syringes into the boiling water and boil them for one hour. Let them cool before
using.
The main point of this technique would be to expose the interior of the jar to as little room air as
possible. Always protect the holes in the lid by placing tin foil or sterile surgical tape over the holes
before and after this procedure.
Email: [email protected]
Site Sponsors