4/6/2020
Biosafety Training
Module 5: Personal Protective
Equipment
University Safety & Assurances
Biological Safety Program
PPE Selection Risk • Determine what needs to be protected
and Use Assessment after all other controls are implemented
• Dress appropriately
Dress to • Minimum: lab coat, safety glasses,
gloves
protect • Additional requirements determined by
risk assessment
Have and • If you don’t know how to don/ doff PPE,
SOP…. And probably won’t use it correctly for lab
work
USE IT
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Lab Coats
• Disposable lab coats or tie back gowns recommended
• If using cloth, you are required to have a routine laundering
service in-place for your laboratory if working in BSL-2.
• Lab Coats are not to leave the labs- do not wear a lab coat into
your office, a cafeteria/ break room, conference rooms, across
campus, etc.
Eye/ Face/ Mucous Membrane Protection
• Refer to OSHA: 29 CFR 1910.133
• All safety glasses must meet ANSI Z87-
2003.
• Safety glasses are to be worn at all times
in the laboratory, unless there is a splash
risk, in which case splash goggles are to
be worn.
• Face shields may be used to protect
whole face. However, eye protection must
still be worn under the face shield.
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Gloves
• Hand protection (29 CFR
1910.138): Appropriate gloves
must be worn for activities
involving potentially infectious
materials.
• When to wear them: at all times
when working in labs.
• Dispose of used gloves as
biohazardous waste when
handling biohazardous
materials.
• Never wash or reuse gloves.
Respiratory Protection
• Respiratory protection may be
necessary when engineering
controls cannot control
airborne exposures.
• Must be worn when working
with RG3 agents.
• For more information, visit:
https://uwm.edu/safety-
health/respiratory/
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Donning PPE
Gloves (if Respiratory
Boot Lab Coat/ Protection
Hand putting on Eye PAPR hood
covers Gown/ Gloves
Hygiene 2 pairs (as Protection (optional)
(optional) Coveralls
only) needed)
Doffing PPE
Gloves Boot covers
Alcohol- Lab Coat/ Gloves (if
Eye Respiratory (if leaving a Hand
based hand (1st pair Gown/ wearing 2nd
Protection Protection BSL-1 or 2 Hygiene
sanitizer only) Coveralls pair)
lab)
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Glove Removal
PPE Disposal
• Decontaminate PPE (gloves, disposable lab coats/ gowns) as necessary.
• Never wear disposable gloves more than one time.
• Gloves can go in general trash if they are not used when handling
anything biohazardous, chemically hazardous, or radioactive. In BSL-2
and higher, should always treat as potentially contaminated and place in
biohazard, not trash.
Think about the person that handles the trash from your lab. NEVER
put anything that may be HAZARDOUS in the general trash- biological
or chemical.
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Wash Your Hands!!
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END
MODULE 5
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