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Microbiology and Parasitology Lab

The document summarizes the key components and functions of a light microscope used in microbiology and parasitology labs. It describes the microscope's illumination system including the illuminator light source and condenser that focuses light on the objective lens. It also outlines the microscope's lens system including the objective lenses, eyepiece, and different types of objectives that provide different magnifications. Finally, it briefly introduces different types of microscopes including dark field, bright field, fluorescence, and electron microscopes that are used for specialized applications.

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33 views1 page

Microbiology and Parasitology Lab

The document summarizes the key components and functions of a light microscope used in microbiology and parasitology labs. It describes the microscope's illumination system including the illuminator light source and condenser that focuses light on the objective lens. It also outlines the microscope's lens system including the objective lenses, eyepiece, and different types of objectives that provide different magnifications. Finally, it briefly introduces different types of microscopes including dark field, bright field, fluorescence, and electron microscopes that are used for specialized applications.

Uploaded by

animumane
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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MICROBIOLOGY AND PARASITOLOGY LAB ILLUMINATION SYSTEM

1. Illuminator – light source


2. Condenser – receive the light ray
MICROSCOPE
* Cone light can be focus on objective
• It is an instrument that enlarges the images * Above condenser iris diaphragm
and allows the visualization of controls the amount of light receive by
morphological cellular details that are too the condenser.
small to be seen by the unaided eyes.
ADJUSTING KNOB
• Joseph Jackson Lister created an essentially
modern light microscope. • Course Adjustment – (lower power)
• Microscope can see smaller parts of the cell. gives general focus and move the stage
up and down
STRUCTURE (The 3 System)
• Fine Adjustments – fine detail (smaller)
1. Main Framework and use with high power
2. Lens System
RHEOSTAT
3. Illumination System
• Help adjust the light intensity and can
BASE – provide support for microscope
rotate clockwise.
ARM – support and hold the magnifying system
POLARISING MICROSCOPE
hold the adjustment system
• Capable of phenomena double
STAGE - place slide or specimen refraction
• To identify cholesterol and collagen
MECHANICAL STAGE – permits or allow the
movement of the stage DARK FIELD MICROSCOPE

LENS SYSTEM • Dark background bright contrast sample


• Unstained sample no dye.
1. Nosepiece – to hold objective in place
2. Objective lenses – consists of system of BRIGHT FIELD
lens and capable of increasing or
decreasing magnification. • Opposite of Dark field
3. X-Y mechanical stage knob FLOURESCENCE MICROSCOPE
4. *Eyepiece *Binocular * Ocular Lens
- Closes to the eye of the observer • Allowed vision of fluorescence dye
- Receive the image * Specimen should be thin
- Has magnifying power (10x or 20x) * Free from bubbles
* Stained
SCANNER
ELECTRON MICROSCOPE
1. Red band – X4 enlarge
2. Yellow band – X10 low power objective • Use to view or attained/ obtained using
3. Blue band – high power objective X40 high resolution using electron bam
4. White Band – Oil immersion X100 • Observation of Viruses.

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