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GCN
‘CMB717 Enrol. No
[BT]
END SEMESTER EXAMINATION : JAN. 2022
PROTEIN ENGINEERING
Time; 3 Hrs. Maximum Marks : 50
Note: Attempt questions from all sections as directed.
SECTION - A (20 Marks)
Attempt any four questions out of five.
Each question carries 05 marks.
1. Discuss the challenges in protein engineering.
2, Explain the differences between the cell surface and
cell-free display. .
3. Describe the role of protein engineering in
multifunctional proteins.
4. Discuss the process of saturation mutagenesis.
5, Explain the principles of Computational protein design.
p.T.O.
(£197) 6. (a) Explain the experimental and c
GCMB717 2
SECTION - B (16 Marks)
Attempt any two questions out of three
Each question carries 08 marks
omputational protein
design and their significance in protein engineering
(4)
(b) Discuss the structure and function of helix-turn-
(4)
helix.
7. Explain the folding process of protein. Also, mention
the comparison between motifs and domains in
proteins. (8)
8. (a) Discuss the application and significance of
engineered Biomimetic proteins. (4)
(b) Explain the classification and significance of amino
acids. (4)
SECTION - C (14 Marks)
(Compulsory)
Discuss the process of protein re-engineering using
non-canonical amino acids along with suitable examples
and elucidation.
(1-197) -
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GCMB717 Enrol
[BT]
END SEMESTER EXAMINATION : APRIL-MAY, 2019
PROTEIN ENGINEERING
Time: 3 Hrs. Maximum Marks : 70
Note: Attempt questions from all sections as directed,
SECTION - A (30 Marks)
Attempt any five questions out of six.
Each question carries 06 marks.
1, Define biomolecules with example and structure.
2. Differentiate protein primary and secondary structure.
3. Discuss in detail methods to study protein evolution
along with its significance.
4. Explain use of motifs in protein engineering and its
benefits with example.
5. Discuss de novo protein design and its advantages.
6. Discuss forward and reverse protein engineering
techniques with example.
p.T.O-
(1795) i. |
GCMB717 2
SECTION - B (20 Marks)
ions out of three,
Attempt any two que.
Each question carries 10 marks.
Describe in detail experimental and computational]
protein design methods and their significance.
Discuss significance and importance of multifunctional
proteins and explain protein engineering methods for
design of therapeutic proteins.
Discuss in detail functional region of proteins and its
importance in protein engineering techniques? Explain
in detail role of ZFN in protein engineering and its
significance?
SECTION - C (20 Marks)
(Compulsory)
Write a workflow explaining techniques and methods
involved in protein design. Discuss in detail any two
case study and its importance. Outline major challenges
and limitations of protein engineering.
