UNIT:- BIOTECHNOLOGY

DPP AND PYQS

1. Organelle/organoid engineering is involved in 6. The sequence that controls the copy number
genetic engineering of the linked DNA in the vector, is termed :
(a) Plasmid (a) Ori site
(b) Mitochondrion (b) Palindromic sequence
(c) Lysosome (c) Recognition site
(d) golgi apparatus (d) Selectable marker

2. In gel electrophoresis, separated DNA fragments 7. A selectable marker is used to:

can be visualized with the help of : (a) Mark a gene on a chromosome for isolation
(a) Ethidium bromide in UV radiation using restriction enzyme.
(b) Acetocarmine in UV radiation (b) Help in eliminating the non-transformants, So
(c) Ethidium bromide in infrared radiation that the transformants can be regenerated.
(d) Acetocarmine in bright blue light (c) Identify the gene for a desired trait in an alien
organism.
3. Bt cotton variety that was developed by the (d) Select a suitable vector for transformation in a
introduction of toxin gene of Bacillus specific crop.
thuringiensis (Bt) is resistant to :
(a) Fungal diseases 8. Given below are four statements pertaining to
(b) Plant nematodes separation of DNA fragments using Gel
(c) Insect predators electrophoresis. Identify the incorrect statements.
(d) Insect pests (i) DNA is negatively charged molecule and so it
is loaded on gel towards the Anode terminal.
4. Identify the wrong statement with regard to (ii) DNA fragments travel along the surface of the
Restriction Enzymes. gel whose concentration does not affect
(a) They cut the strand of DNA at palindromic sites. movement of DNA.
(b) They are useful in genetic engineering. (III) Smaller the size of DNA fragment, larger is the
(c) Sticky ends can be joined by using DNA ligases. distance it travels through it.
(d) Each restriction enzyme functions by inspecting (iv) Pure DNA can be visualised directly by
the length of a DNA sequence. exposing to UV radiation.
(a) (i), (ii) and (iv)
5. The specific palindromic sequence which is (b) (i), (iii) and (iv)
recognized by EcoRl is : (c) (i), (ii) and (iii)
(a) 5’-GGAACC-3’ , 3’-CCTTGG-5’ (d) (ii), (iii) and (iv)
(b) 5’-CTTAAG-3’ , 3’-GAATTC-5’
(c) 5’-GGATCC-3’, 3’-CCTAGG-5’ 9. An enzyme catalysing the removal of
(d) 5’-GAATTC-3’, 3’-CTTAAG-5 nucleotides from ends of DNA is:
(a) Protease
(b) DNA ligase
(c) Endonucleases
(d) Exonucleases
10. Biolistics (gene-gun) is suitable for 16. The DNA fragments separated on an agarose gel
(a) DNA finger printing. can be visualised after staining with:
(b) Disarming pathogen vectors. (a) Acetocarmine
(c) Transformation of plant cells. (b) Aniline blue
(d) Constructing recombinant DNA by joining (c) Ethidium bromide
with vectors. (d) Bromophenol blue

11. The two antibiotic resistance genes on vector 17. A gene whose expression helps to identify
pBR322 are for transformed cell is known as:
(a) Tetracycline and Kanamycin (a) Vector
(b) Ampicillin and Tetracycline (b) Plasmid
(c) Ampicillin and Chloramphenicol (c) Structural gene
(d) Chloramphenicol and Tetracycline (d) Selectable marker

12-Which one of the following equipments is 18. What is the criterion for DNA fragments
essentially required for growing microbes on a movement on agarose gel during gel electrophoresis?
large scale, for industrial production of enzymes? (a) The smaller the fragment size, the farther it
(a) BOD incubator moves
(b) Sludge digester (b) Positively charged fragments move to farther
(c) Industrial oven end
(d) Bioreactor (c) Negatively charged fragments do not move
(d) The larger the fragment size, the farther it moves
13-Following statements describe the
characteristics of the enzyme Restriction 19. Which of the following is not a feature of
Endonuclease. Identify the incorrect statement. the plasmids?
A. The enzyme cuts DNA molecule at identified (a) Independent replication
position within the DNA. (b) Circular structure
B. The enzyme binds DNA at specific sites and (c) Transferable
cuts only one of the two strands. (d) Single stranded
C. The enzyme cuts the sugar-phosphate
backbone at specific sites on each strand. 20. The taq polymerase enzyme is obtained from
D. The enzyme recognizes a specific palindromic (a) Thermus aquaticus
nucleotide sequence in the DNA. (b) Thiobacillus ferroxidans
(c) Bacillus subtilis
14-Which of the following is commonly used as a (d) Pseudomonas putida
vector for introducing a DNA fragment in
human lymphocytes? 21. Which of the following is a restriction
A. Retrovirus endonuclease?
B. Ti plasmid (a) Hind II
C. pBR 322 (b) Protease
D. Lambda phage (c) DNase I
(d) RNase
15. The correct order of steps in Polymerase Chain
Reaction (PCR) is : 22. The cutting of DNA at specific locations
(a) Extension, Denaturation, Annealing became possible with the discovery of :
(b) Annealing, Extension, Denaturation (a) Probes
(c) Denaturation, Annealing, Extension (b) Selectable markers
(d) Denaturation, Extension, Annealing (c) Ligases
(d) Restriction enzymes
23. The DNA molecule to which the gene of interest 29. For transformation, micro-particles coated with
is integrated for cloning is called: DNA to be bombarded with gene gun are made up of:
(a) Vector (a) Silver or Platinum
(b) Template (b) Platinum or Zinc
(c) Carrier (c) Silicon or Platinum
(d) Transformer (d) Gold or Tungsten

24. An analysis of chromosomal DNA using the 30. A single strand of nucleic acid tagged with a
southern hybridization technique does not use: radioactive molecule is called:
(a) Electrophoresis (a) Vector
(b) Blotting (b) Selectable marker
(c) Autoradiography (c) Plasmid
(d) PCR (d) Probe

25. Which vector can clone only a small fragment 31. Which one of the following represents a
of DNA? palindromic sequence in DNA?
(a) Bacterial artificial chromosome (a) 5’-GAATTC – 3’ , 3’-CTTAAG-5’
(b) Yeast artificial chromosome (b) 5’-CCAATG-3’ , 3’-GAATCC-5’
(c) Plasmid (c) 5’-CATTAG-3’, 3’-GATAAC-5’
(d) Cosmid (d) 5’-GATACC – 3’ 3’-CCTAAG-5’

26. During the process of isolation of DNA, chilled 32. Which one of the following techniques made it
ethanol is added to : possible to genetically engineer living organism
(a) Remove proteins such as histones (a) Recombinant DNA techniques
(b) Precipitate DNA (b) X-ray diffraction
(c) Break open the cell to release DNA (c) Heavier isotope labelling
(d) Facilitate action of restriction enzymes (d) Hybridization

27. The colonies of recombinant bacteria appear 33. There is a restriction endonuclease called EcoRI.
white in contrast to blue colonies of What does “co” part in it stand for?
non- recombinant bacteria because of: (a) Colon
(a) Insertional inactivate of alphaga- lactosidase in (b) Coelom
non-recombinant bacteria (c) Coenzyme
(b) Insertional inactivation of alpha- galactosidase (d) Coli
in recombinant bacteria
(c) Inactivation of glycosidase enzyme in 34. Which one of the following is used as vector for
recombinant bacteria cloning genes into higher organisms?
(d) Non-recombinant bacteria containing (a) Baculovirus
beta-galactosidase (b) Salmonella typhimurium
(c) Rhizopus nigricans
28. Genes of interest can be selected from a (d) Retrovirus
genomic library by using
35. Which one of the following palindromic base
(a) Restriction enzymes
sequences in DNA can be easily cut at about the
(b) Cloning vectors
middle by some particular restriction enzyme?
(c) DNA probes
(a) 5’...CGTTCG.3’ 3..ATGGTA....5’
(d) Gene targets
(b) 5’..GATATG..3’ 3'.СТАСТА.. 5
(c) 5’ .. GAATTC …3’ 3’.CTTAAG..5’
(d) 5’. CACGTA..3’ 3'CTCAGT .. 5’
36. DNA or RNA segment tagged with a radioactive 43. What is antisense technology?
molecule is called : (a) A cell displaying a foreign antigen used
(a) Vector for synthesis of antigens
(b) Probe (b) Production of somaclonal variants in tissue cultures
(c) clone (c) When a piece of RNA that is complementary in
(d) Plasmid sequence is used to stop-expression of a specific gene
(d) RNA polymerase producing DNA
37. Restriction endonucleases are enzymes which :
(a) Make cuts at specific positions within the 44. Chromosomes in a bacterial cell can be 1-3 in
DNA molecule number and
(b) Recognize a specific nucleotide sequence for (a) Can be circular as well as linear within the same cell
binding of DNA ligase (b) Are always circular
(c) restrict the action of the enzyme DNA (c) are always linear
polymerase (d) Can be either circular or linear, but never both
(d) remove nucleotides from the ends of the DNA within the same cell
molecule
45. In bacteria, plasmid is :
38. Stirred tank bioreactors have been designed for (a) Extra-chromosomal material
(a) addition of preservatives to the product (b) Main DNA
(b) purification of the product (c) non-functional DNA
(c) ensuring anaerobic conditions in the culture (d) Repetitive gene
vessel
(d) availability of oxygen throughout the process 46. Introduction of food plants developed by
genetic engineering is not desirable because :
39. Polyethylene glycol method is used for : (a) Economy of developing countries may suffer
(a) biodiesel production (b) These products are less tasty as compared to
(b) seedless fruit production the already existing products
(c) energy production from sewage (c) This method is costly
(d) gene transfer without a vector (d) There is danger of entry of viruses and toxins
with introduced crop
40 in transfer of foreign DNA into crop plants?
(a) Meloidogyne incognita 47. Two bacteria found to be very useful in genetic
(b) Agrobacterium tumefaciens engineering experiments are
(c) Penicillium expansum (a) Escherichia and Agrobacterium
(d) Trichoderma harzianum (b) Nitrobacter and Azotobacter
(c) Rhizobium and Diplococcus
41. The linking of antibiotic resistance gene with (d) Nitrosomonas and Klebsiela
the plasmid vector became possible with
(a) DNA ligase 48 The plasmids present in the bacterial cells are
(b) Endonucleases (a) Circular double helical DNA molecules
(c) DNA polymerase (b) Circular double helical RNA molecules
(d) Exonucleases (c) linear double helical DNA molecules
(d) Linear double helical RNA molecules.
42. Gel electrophoresis is used for :
(a) Cutting of DNA into fragments 49. Choose the correct pair from the following :
(b) Separation of DNA fragments according to (a) Polymerases – Break the DNA into fragments
their size (b) Nucleases Separate the two strands of DNA
(c) construction of recombinant DNA by joining (c) Exonucleases - Make cuts at specific positions
with cloning vectors within DNA
(d) Isolation of DNA molecule (d) Ligases – Join the two DNA molecules
 57. Golden rice is a genetically modified crop plant
50. DNA precipitation out of a mixture of where the incorporated gene is meant for
biomolecules can be achieved by treatment with : biosynthesis of:
(a) Isopropanol (a) Vitamin C
(b) Chilled ethanol (b) Omega 3
(c) Methanol at room temperature (c) Vitamin A
(d) Chilled chloroform (d) Vitamin B

51. In RNAi, the genes are silenced using : 58. Pollen tablets are available in the market for:
(a) Ds-DNA a. In vitro fertilization
(b) Ds – RNA b. Breeding programmes
(c) ss-DNA c. Supplementing food
(d) SS – RNA d. Ex situ conservation

52. Which of the following is true for Golden rice? 59. Which of the following Bt crops is being grown
a. It is vitamin A enriched, with a gene from in India by the farmers?
daffodil. a. Cotton
b. It is pest resistant, with a gene from Bacillus b. Brinjal
thuringiensis. c. Soybean
c. It is drought tolerant, developed using d. Maize
Agrobacterium vector.
d. It has yellow grains, because of a gene 60. RNA interference involves :
introduced from a primitive variety of rice. a. Synthesis of mRNA from DNA
b. Synthesis of cDNA from RNA using reverse
53. What triggers activation of protoxin to active transcriptase
Bt toxin of Bacillus thuringiensis in boll worm? c. Silencing of specific mRNA due to
a. Body temperature complementary RNA
b. Moist surface of midgut d. Interference of RNA in synthesis of DNA
c. Alkaline pH of gut
d. Acidic pH of stomach 61. Consumption of which one of the following
foods can prevent the kind of blindness associated
54. Which part of the tobacco plant is infected by with vitamin ‘A’ deficiency?
Meloidogyne incognita? a. ‘Flavr Savr’ tomato
(a) Flower b. Canolla
(b) Leaf c. Golden rice
(c) Stem d. Bt-Brinjal
(d) Root
62. Tobacco plants resistant to a nematode have
55. The crops engineered for glyphosate are been developed by the introduction of DNA that
resistant/ tolerant to : produced (in the host cells) :
a. Bacteria a. Both sense and anti-sense RNA
b. Insects b. A particular hormone
c. Herbicides c. an antifeedant
d. Fungi d. A toxic protein

56. In Bt cotton, the Bt toxin present in plant tissue 63. The most common substrate used in
as pro-toxin is converted into active toxin due to: distilleries for the production of ethanol is ::
a. Acidic pH of the insect gut a. Corn meal
b. Action of gut micro-organisms b. Soya meal
c. Presence of conversion factors in insect gut c. ground gram
d. Alkaline pH of the insect gut d. Molasses
64. The process of RNA interference has been used 69. Some of the characteristics of Bt cotton are:
in the development of plants resistant to : a. Long fibre and resistance to aphids
a. Nematodes b. Medium yield, long fibre and resistance to
b. Fungi beetle pests
c. Virus c. high yield and production of toxic protein
d. Insects crystals which kill dipteran pests
d. High yield and resistance to bollworms
65. Silencing of mRNA has been used in
producing transgenic plants resistant to: 70. What is true about Bt toxin ?
a. Bollworms a. Bt protein exists as active toxin in the Bacillus
b. Nematodes b. The activated toxin enters the ovaries of the
c. White rust pest to sterilise it and thus prevent its
d. Bacterial blight multiplication.
c. The concerned Bacillus has antitoxins.
d. The inactive protoxin gets converted into
66. Consider the following statements (A-D)
active form in the insect gut.
about organic farming:
i. Utilizes genetically modified crops like Bt cotton
71. The bacterium Bacillus thuringiensis is
ii. Uses only naturally produced inputs like
widely used in contemporary biology as :
compost
a. Insecticide
iii. Does not use pesticides and urea
b. Agent for production of dairy products
iv. Produces vegetables rich in vitamins and
c. source of industrial enzyme
minerals
d. Indicator of water pollution

Which of the above statements are correct? 72. Main objective of production/use of herbicide
(a) (B), (C) and (D) resistant GM crops is to :
(b) (C) and (D) only a. Eliminate weeds from the field without the use
(c) (B) and (C) only of manual labour
(d) (A) and (B) only b. eliminate weeds from the field without the use
of herbicides
67. Bacillus thuringiensis forms protein crystals c. encourage eco-friendly herbicides
which contain insecticidal protein that d. Reduce herbicide accumulation in food
a. Binds with epithelial cells of midgut of the articles for health safety
insect pest ultimately killing it
b. Is coded by several genes including the 73. Cry endotoxins obtained from Bacillus
gene cry thuringiensis are effective against
c. Is activated by acid pH of the foregut of the a. Mosquitoes
insect pest. b. Flies
d. Does not kill the carrier bacterium which is c. nematodes
itself resistant to this toxin d. Boll worms

68. The genetically-modified (GM) brinjal in India 74. The name of Norman Borlaug is associated with:
has been developed for : a. White revolution
a. Insect-resistance b. Green revolution
b. Enhancing shelf life c. yellow revolution
c. enhancing mineral content d. Blue revolution
d. Drought-resistance
75. Bacillus thuringiensis (Bt) strains have been
used for designing novel:
a. Biofertilizers 81. Which one of the following vectors is used to
b. Bio-metallurgical techniques replace the defective gene in gene therapy?
c. Bio-mineralization processes a. Ti plasmid
d. Bioinsecticidal plants b. Adenovirus
c. Cosmid
76. One of the most important reasons why wild d. Ri plasmid
plants should thrive is that they are
good sources of : 82. The first clinical gene therapy was given
a. Highly nutritive animal feel for treating :
b. Unsaturated edible oils a. Chicken pox
c. Genes for resistance to disease and pests b. Diabetes mellitus
d. Very rare and highly sought after fruits of c. Rheumatoid arthritis
medical importance d. Adenosine deaminase deficiency

77. Two microbes found to be very useful in 83. Continuous addition of sugars in ‘fed batch’
genetic engineering are : fermentation is done to:
(a) Vibrio cholerae and a tailed bacteriophage
a. Produce methane
(b) Diplococcus sp. And Pseudomonas sp.
b. Obtain antibiotics
(c) Crown gall bacterium and Caenorhabditis
c. purify enzymes
elegans
d. Degrade sewage
(d) Escherichia coli and Agrobacterium
tumefaciens
84. Consider the following statements about
biomedical technologies?
78. Which of the following statements is
1. During open heart surgery blood is circulated in
not correct?
the heart-lung machine
a. The proinsulin has an extra peptide
2. Blockage in coronary arteries is removed
called C-peptide.
b. The functional insulin has A and B chains by angiography
linked together by hydrogen bonds. 3. Computerised axial tomography BO shows
c. Genetically engineered insulin is produced detailed internal structure as seen in a section
in E. Coli. of body
d. In man insulin is synthesised as a proinsulin 4. X-ray provides clear and detailed images of
organs like prostate glands and lungs .
79. Which of the following features of genetic
code does allow bacteria to produce human Which two of the above statements are correct?
insulin by recombinant DNA technology? a. 2 and 4
a. Genetic code is not ambiguous b. 3 and 4
b. Genetic code is redundant c. 1 and 3
c. Genetic code is nearly universal d. 1 and 2
d. Genetic code is specific
85. Ultrasound of how much frequency is
80. The first human hormone produced by beamed into human body for sonography?
recombinant DNA technology is: a. 15-30 MHZ
a. Insulin b. 115 MHZ
b. Estrogen c. 45-70 MHZ
c. Thyroxin d. 30-45 MHZ.
d. Progesterone
86. Maximum application of animal cell culture
technology today is in the production of :
a. Edible proteins
b. Insulin
c. interferons
d. Vaccines

87. What is true for monoclonal antibodies?

a. These antibodies obtained from one parent
and for one antigen
b. These antibodies obtained from parent and
for two antigen
c. These antibodies obtained from one parent
and for many antigen
d. These antibodies obtained from many
parents and for many antigens

88. Genetically engineered bacteria been

successfully used in the commercial
have production of :
a. Human insulin
b. Testosterone
c. Thyroxine
d. Melatonin

89. Exploration of molecular, genetic and species

level diversity for novel products of economic
importance is known as:
a. Bioprospecting
b. Biopiracy
c. Bioenergetics
d. Bioremediation

90. Exploitation of bioresources of a nation by

multinational companies without authorisation
from the concerned country is referred to as :
a. Biowar
b. Bioweapon
c. Biopiracy
d. Bioethics
1. A 26. B 51. B 76. C
2. A 27. D 52. A 77. D
3. D 28. C 53. C 78. B
4. C 29. D 54. D 79. C
5. D 30. D 55. C 80. A
6. A 31. A 56. D 81. B
7. B 32. A 57. C 82. D
8. A 33. D 58. C 83. C
9. D 34. D 59. A 84. C
10. C 35. C 60. C 85. B
11. B 36. B 61. C 86. A
12. D 37. A 62. A 87. A
13. B 38. D 63. D 88. A
14. A 39. D 64. A 89. A
15. C 40. B 65. B 90. C
16. C 41. A 66. C
17. D 42. D 67. A
18. A 43. C 68. A
19. D 44. B 69. D
20. A 45. A 70. D
21. A 46. D 71. A
22. D 47. A 72. A
23. A 48. A 73. D
24. D 49. A 74. B
25. C 50. B 75. D