Taurine
Standard solution
Weigh accurately about 250 mg of taurine working standard into clean, 50 mL volumetric
flask. Add 30 mL of diluents and sonicate for 10 minutes. Make up the volume to 50 mL with
the diluent and mix well. Dilute 5 mL of this solution to 50 mL with the diluent.
Test solution
Weigh and crush 20 tablets to a fine powder. Weigh accurately about 420mg of powdered
tablet into clean, 50 mL volumetric flask. Add 30 mL of diluent and sonicate for 10 minutes.
Make up the volume to 50 mL with the diluent, mix well and filter. Dilute 5 mL of the filtrate
to 50 mL with the diluents.
Preparation of Mobile phase
Mobile phase A
Weigh accurately about 2.72 g of anhydrous sodium acetate and dissolve it in 1000 mL of
water. Add 0.18 mL of triethylamine and adjust the pH to 7.2 using dilute acetic acid and
then add 3.0 mL of tetrahydrofuran. Mix well and filter and degas it.
Mobile phase B
Weigh accurately about 2.72 g of anhydrous sodium acetate and dissolve it in 200 mL of
water. Adjust the pH to 7.2 using dilute acetic acid and then add 400 mL of acetonitrile and
400 mL of methanol. Mix well and filter and degas it.
S.No Contents taurine
1. Flow rate 1mL/ min
2. Column osmosilC18MS-11 (250×4.6mm,5μ)
3. Column temperature 400C
4. Injection volume 10μL
5. Mobile phase Mix equal volume of mobile phase A and B
6. Diluent Mix equal volume of mobile phase A and B
7. Wave length 338nm
