Group Number: 1 Section: STEM 12-12
Group Members: Gardose, Lemniel Gift I., Garque, Alexandria B. Date: September 21, 2024
Gabayoyo, Pia Abigail, Lagradante, Jamila Aylah, Moscoso, Maribel B., Luable, Jeopherdon T., Arroz, Billy Justin
Lab Activity 2
The Cell 15 minutes
Let’s Review
Cells are the fundamental units of structure and function in all living organisms. As such, one of
the principles of cell theory states that all living organisms are composed of at least one cell.
Although microscopic in size, cells are composed of structures that are complex in function and
different subcellular parts which we call as organelles. The cell and its organelles cannot be
seen through the naked eye that is why one must know and understand how to manipulate a
microscope to use it in observing the cell.
Objectives
At the end of this lesson, you should be able to do the following:
● Prepare a wet mount slide.
● Manipulate a microscope for focusing on a specimen.
● View cells under the microscope using a prepared slide.
Materials
● Potato, tomato, bell pepper, Rhoeo discolor, cut-out letter “e”
● dissecting knife/scalpel
● glass slide
● dropper
● water
● coverslip
● Iodine Solution
● compound microscope
Procedure
1. Get a piece of the specimen and slice it into very thin pieces using the scalpel or
dissecting knife. Make the sections of the specimen as thin as possible.
Reminder
Be careful when handling the scalpel or dissecting knife to avoid injury. Make
sure you also adjust the knobs of the microscope carefully to avoid slide
breakage.
2. Place the piece of the thin slice of specimen on the glass slide. Prepare a wet mount by
dropping some water into the slide where the specimen slices are. Cover it with a
coverslip. Make sure that there are no air bubbles under the coverslip.
3. Observe the specimen under the microscope.
a. Place the slide on the microscope stage. Use the coarse adjustment knob to
focus the specimen on the scanner or low power objective.
b. Once focused on lower power, switch to the high power objective and use the
fine adjustment knob to focus.
4. Draw the cell that you have observed and label its significant parts on the box provided
in Fig. 1.1.
5. In the absence of sufficient microscopes and slides, you may view the following video:
Data and Results
Guide Questions
Answer the following questions.
1. What are the significant subcellular organelles that you have seen when observing the
cells under the microscope?
2. What organelle/s can be found in the plant cell that cannot be found in a typical animal
cell?
3. What is the total magnification of the specimen viewed under the High Power Objective if
the magnification of the ocular lens is 10x and the magnification of the HPO is 40x?
4. Why are cells considered the basic unit of life?
Conclusion
Bibliography
Laboratory Handbook for Teachers. National Biology Support Service, n.d.
Burran, Susan, and David DesRochers. Principles of Biology I Laboratory Manual. University
System of Georgia, 2015.
