Republic of the Philippines
Department of Education
Region VII, Central Visayas
DIVISION OF CARCAR CITY
P. Nellas St., Poblacion III, Carcar City, Cebu
Instructional Plan
IP No.10 week 3 day 4 Learning Area: SCIENCE Grade Level: Quarter: Duration:
Date: __________ 7 2 60mins.
Learning Code: S7LT-IIb-
Competency/ies Focus specimen using the compound microscope 2
Key Concepts/
Understanding to be Skill Demonstration on Manipulating Microscope.
Developed:
1. Objectives
Knowledge Recognize the parts of the microscope and it’s functions
Skills Manipulate the microscope properly.
Attitudes Demonstrate carefulness in handling the microscope.
Values Practice patience in focusing the specimen in the microscope.
2. Content/ Topic Manipulating the Microscope
3. Learning
Resources/ Materials/ Microscope
Equipment
4. Procedures
4.1. Introductory Review the previous topic.
Activity Ask the students to compare the image of the onion skin seen under the low power
(suggested activity) objective and high power objective.
(5 minutes)
4.2. Activity Individual Activity: Manipulating Microscope
10 mins.
4.3. Analysis Question/s:
How does a microscope work?
4.4. Abstraction: Discuss the proper ways of manipulating microscope including the do’s and don’ts.
4.5. Application: Oral Recitation
Assessment Matrix
Level of What will I How will I How will I Score?
Assessment Assess? Assess?
Observation Skill Students will Accuracy – 70
5. Assessment Demonstration demonstrate Speed - 30
(58 minutes) on manipulating on how to
the microscope manipulate
the
microscope
and focus
specimen
clearly.
6. Assignment
(1 minute)
Preparing for the Read about characteristics of life.
new/continuation of Define life.
the lesson.
7. Wrap-Up/
Concluding Activity -
Prepared by: Danao City Division
Enhanced by:
-
Fe U. Barasona Gelacio C. Babao Sr. MNHS
-
Lerio A. Garzo Ocaňa National High School
-
Cindy V. Navarro Ocaňa National High School
-
Sonia C. Abanto Gelacio C. Babao Sr. MNHS
-
Activity # 3
Investigating Plant Cells
Objectives:
In this activity, you should be able to:
1. Prepare a wet mount.
2. Stain plant cell. ( onion skin)
3. Draw onion cells as seen through the light microscope.
4. Explain the role of microscope in cell study.
Materials Needed
Dropper , tissue paper
Cover slip , iodine solution
Glass slide , light microscope
Onion bulb scale , forceps or tweezers
Scalpel or sharp blade , 50-ml beaker with tap water
Procedure:
1. Prepare the onion scale by the following steps indicated below. Use the transparent skin from the inner
surface of the onion scale.
A. Cut an onion B. Remove one of the
fleshy “scale” leaves
into quarters
C. Snapping the “leaf” backward
usually produces a ragged piece
epidermis.
E. Gently lower a cover slip to prevent
trapping air bubbles.
D. Remove a small piece of epidermis Examine with your microscope. Add
and spread it smoothly in a drop of water more water to the edge
on slide. of the coverslip with an eyedropper if
the slides begins to dry.
Figure 1 G. Drew the stain under by touching
F. Add one or two drops of methylene
absorbent paper
blue or Janus Green stain to the edge
to the opposite side of the cover slip.
of the cover
p.
2. Following the procedure on how to make a wet mount described in “ How to Use The Light Microscope”, to
prepare one using the transparent onion skin form Step 1. Remember to place it on the glass slide with the
inner surface ( non-waxy side) facing up. Check too that the onion skin is not folded or wrinkled.
3. Examine the onion skin slide under the low power objective (LPO).
Caution: Do not tilt the microscope.
Q1. How much are these onion cells magnified?
Ans: _____________________________________________________________________________________
Q2. In this case, why is it not good to tilt the microscope?
Ans.:____________________________________________________________________________________
4. Shift to the high power objective ( HPO).
Remember: Raise the objectives a little and look to the side while changing objectives!
5. Remove the slide from the stage. You can now stain the onion cells with iodine solution.
Be careful not to spill it on your skin and clothing!
6. Using a dropper, place one or two drops of iodine solution along one edge of the cover slip. Place a piece of
tissue paper on the other edge of the cover slip. The tissue paper will absorb the water, iodine solution
spreads out under the cover slip until the whole specimen is covered with stain.
7. Examine the stained onion cells under the LPO and HPO.
Q3. Did you observe any change in the image of onion cells before and after staining?
Ans.: ________________________________________________________________________________________
Q4. How did the iodine solution affect the image of the onion cells?
Ans.: _______________________________________________________________________________________
Q5. Of what importance is the contribution of the microscope in the study of cells?
Ans.: ______________________________________________________________________________________
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