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Question Bank-Biotechnology Applications

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410 views4 pages

Question Bank-Biotechnology Applications

Uploaded by

Aaaa_raaaa
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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OUR OWN HIGH SCHOOL.

ALWARQA’A
GRADE 12 BIOLOGY
CHAPTER – BIOTECHNOLOGY-APPLICATIONS
Multiple Choice Questions

1. Cry I endotoxins obtained from Bacillus thuringiensis are effective against


(a) nematodes (c) mosquitoes
(b) bollworms (d) flies
2. Human insulin is being commercially produced from a transgenic species of
(a) Rhizobium (c) Escherichia
(b) Saccharomyces (d) Mycobacterium
3. Which one of the following is commonly used in transfer of foreign DNA into crop
plants?
(a) Meloidogyne incognita (c) Penicillium expansum
(b) Agrobacterium tumefaciens (d) Trichoderma harzianum
4. Genetic engineering has been successfully used for producing
(a) transgenic mice for testing safety of polio vaccine before use in humans
(b) transgenic models for studying new treatments for certain cardiac diseases
(c) transgenic cow-Rosie which produces high fat milk for making ghee
(d) animals like bulls for farm work as they have superpower
5. C-peptide of human insulin is
(a) a part of mature insulin molecule
(b) responsible for formation of disulphide bridges
(c) removed during maturation of proinsulin to insulin
(d) responsible for its biological activity
6. GEAC stands for
(a) Genome Engineering Action Committee
(b) Ground Environment Action Committee
(c) Genetic Engineering Approval Committee
(d) Genetic and Environment Approval committee
7. The site of production of ADA in the body is
(a) erythrocytes (c) blood plasma
(b) lymphocytes (d) osteocytes
8. Bt corn has been made resistant from corn borer disease by introduction of the gene
(a) cryIAb
(b) ampR
(c) cryIIAb
(d) trp
9. ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of
ADA?
(a) Adenosine deoxyaminase
(b) Adenosine deaminase
(c) Aspartate deaminase
(d) Arginine deaminase
10. Biopiracy means
(a) use of biopatents
(b) thefts of plants and animals
(c) stealing of bioresources
(d) exploitation of bioresources without authentic permission
In the following questions a statement of assertion follow
ed by a statement of reason is given. Choose the correct answer out of the following choices.
(a) Assertion and reason both are correct statements and reason is correct explanation for
assertion.
(b) Assertion and reason both are correct statements, but reason is not correct explanation
for assertion.
(c) Assertion is correct statement, but reason is wrong statement.
(d) Assertion is wrong statement, but reason is correct statement.
11. Assertion: Genetic engineering is mainly involved in production of transgenic animals
that produce proteins.
Reason: Transgenic plants can be obtained by combination of tissue culture and genetic
engineering.
12. Assertion: The first gene therapy was given for ADA deficiency.
Reason: The normal gene for ADA was delivered to patient’s cells using retroviral vector.
13. Assertion: ‘Cry’ proteins are named so because they are crystal proteins.
Reason: ‘Cry’ proteins solubilise in alkaline pH of the insect’s gut and activate Bt toxin
14. Assertion: Patents are granted by government to an inventor.
Reason: Patent prevents other from commercial use of an invention.
15. Assertion: Indian Patents Bill takes cases of biopiracy in consideration.
Reason: Biopiracy is the use of bioresources by multinational companies without proper
authorisation from concerned persons and countries.
Case Based Questions
16. Refer to the diagram of maturation of proinsulin into insulin to answer the following
questions
[3]
(i) How are two short polypeptide chains of insulin linked together?
(ii) State the role of C-peptide in human insulin.
(iii) Mention the chemical change that proinsulin undergoes, to be able to act as mature
insulin.

Short Answer Questions [1 mark]


17. Mention the source organism of the gene cryIAc and its target pest.
18. Mention the chemical change that proinsulin undergoes, to be able to act as mature
insulin.
19. Write the two uses of PCR technique in diagnosis.
20. What was the speciality of the milk produced by the transgenic cow Rosie?
21. State the cause of adenosine deaminase enzyme deficiency.
22. A boy has been diagnosed with ADA deficiency. Suggest any one possible treatment.
23. What are transgenic animals? Give an example.
24. What is biopiracy?
Short Answer Questions [2 marks]
25. Highlight any four advantages of genetically modified organisms (GMOs).
26. Expand GMO. How is it different from a hybrid?
27. Name the insect pest that is killed by the products of cryIAc gene. Explain how the gene
makes the plant resistant to the insect pest.
28. A corn farmer has perennial problem of corn-borer infestation in his crop. Being
environmentally conscious he does not want to spray insectisides. Suggest solution
based on your knowledge of biotechnology. Write the steps to be carried out to achieve
it.
29. Differentiate between gene therapy and gene cloning.
30. Why is the introduction of genetically engineered lymphocytes into an ADA deficiency
patient not a permanent cure? Suggest a possible permanent cure.
Long Answer Questions–I [3 marks]
31. Describe the various stages involved in gene transfer for the commercial production of
human insulin by Eli Lilly.
32. Biotechnology has helped farmers to get pest resistant cotton crops. Explain the
technique adopted along with its mode of action. (Mention six points)
33. How did the process of RNA interference help to control the nematode from infecting
roots of tobacco plants? Explain.
34. A person is born with a hereditary disease with a weakened immune system due to
deficiency of an enzyme. Suggest a technique for complete cure for this disease, identify
the deficient enzyme and explain the technique used for cure.

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