The document outlines an experiment for estimating the amount of hemoglobin in blood using Sahli's method, which involves visual color comparison after treating blood with dilute hydrochloric acid. It describes the necessary equipment, including a hemoglobinometer, and the principle behind the method, which relies on the formation of acid hematin. The document also mentions the importance of hemoglobin as a protein rich in iron that is crucial for oxygen transport in the blood.
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Hemoglobin Count Experiment
The document outlines an experiment for estimating the amount of hemoglobin in blood using Sahli's method, which involves visual color comparison after treating blood with dilute hydrochloric acid. It describes the necessary equipment, including a hemoglobinometer, and the principle behind the method, which relies on the formation of acid hematin. The document also mentions the importance of hemoglobin as a protein rich in iron that is crucial for oxygen transport in the blood.
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‘good AnalySis/Haematology
root
EXPERIMENT No,
estimate amount of hag
Learning Objectives:
jeemoglobin in blood, 1
alldiseases of blood. This testis tra
sits ability to combine with ox. ies of haemoglobin such
faemogiobin and the ability op rount of iron in each gram of
Resorption bands at specitie y, x of haemoglobin to give typical
laemoglobinometer.
rement of amount of
ed onthe 'S @ part of routine tests in
recial character
‘YBN, presence
of known am
Requirements:
“rilized needle, cotton, Santi
Chemicals: Human blood, alcohol, distil
Principle: Haemoglobin (Hb) is the most
ed color to it. It is a com
' haemoglobinometer apparatus
ed water and | /10N HCL.
Mportant pigment of the blood imparting
Plex protein rich in iron that combines eer O, forming
carbamino compound,
The amount of this important component of blood can easily be estimated in the
laboratory by visual color comparison. The methods of Ib estimation are ahli, Haldane
and Talliqvist. Out of these, Sahli’s method is the most acceptable. The principle of
Visual color comparison method is that, blood when treated with dilute hydrochloric acid
Produc brown color due to the formation of acid haematin. This brown color is then
compared with the standard brown colored solutions of acid haematin produced by similar
tteatment of blood of known hemoglobin percentage. The value obtained gives the amount
oFhemogiobin in gm % in the desired blood. 1
ir : The hemoglobin apparatus (Figure
Ei eieeae eM RE cases erseitn nied es
i hs rere ee {us comes in a wooden box and it consists
Suhli’s Hacmoglobinometer. The entire apparatus
ee (Figure 2A) with a mark of 20 cubic mm (0.02 ml)
ce aaa eect skin te blood from be source.
Has ag el, a'sching rubber tube and a mouth
The other end of this pipette is connect
Piece. igure 2B) is an experimental tube and is graduated
2. A haemoglobinom: ieee in grams per 100 ml on the other side. The lowest
a a , 2 gm % respectively.
Tigure 2) which acts asa stirrer eo
lored glass tubes/ro‘
ace Se ar oplobinometer ube paced in
them. Haemoglobin
d provides the necessary contrast for easy
tal and standard tubes. |ysiaomatology
s ee
Figure 1: Pholopaph ol Hemoslcinometer Apart fled above the solution only
RELEVANT QUESTIONS
‘of Sahll's method forthe estimation of
method for the estimation of haemogt
{sestimated by Sahll's method?
ee in and reduced hemoglobin. However,
not detected.
carbaminohacmoglobin,
pin value?
