Figure 3: p53-dependent attrition of neural progenitors in Cep63-deficient embryos.

(a) Strong induction of p53 in the cortex of Cep63^T/T embryos compared with WT at E14.5 (scale bar, 50 μm). (b) Scoring of p53-positive cells in a (n=2 animals per genotype). (c) Slightly increased levels of γH2AX can be visualized in the E14.5 cortex of Cep63^T/T embryos compared with WT (scale bar, 50 μm). (d) Quantification of γH2AX-positive cells in c (n=1 animal per genotype). (e) Dissected p60 brains (upper panel) and representative coronal sections from Cep63^T/T and Cep63^T/Tp53^−/−mice (lower panel). (f) Representative examples illustrating relative p60 motor cortex thickness in mice of the indicated genotype (scale bar, 0.2 mm). (g) Measurement of cortex thickness from sections of the motor or visual cortex with colours corresponding to genotypes in f (n=3, 3, 2, 3, 2 and 2 animals used per genotype, respectively). (h) Quantification of TUNEL staining (n=6, 10, 4 and 4 sections per animal were scored, sections from 2, 3, 1 and 1 animals per genotype were used, respectively). (i) Misplacement of neural/stem cell progenitors (Sox2 positive) in Cep63^T/T and Cep63^T/Tp53^−/−animals (scale bar, 50 μm). (j) Rescue of misplaced progenitors in Cep63^T/Tp53^−/−animals compared with Cep63^T/T (n=5, 3 and 3 animals per genotype, respectively). All graphics with error bars are presented as the average plus s.d. Asterisks denote statistical significance (*P value <0.05, **P value <0.01 and ***P value<0.001) determined by the unpaired two-way Student’s t-test (b,g,h,j).