Global Regulation of Nucleotide Biosynthetic Genes by c-Myc
Figure 2
Nucleotide biosynthetic genes are responsive to MYC induction in human B cells in vitro.
The expression of the nucleotide synthetic genes is temporally responsive to Myc induction. The inset shows an immunoblot of Myc expression in P493-6 cells withdrawn from tetracycline. Bar graphs represent mRNA expression of each indicated nucleotide synthetic gene relative to 18S rRNA control as determined by triplicate real-time PCR reactions (with less than 5% error from the mean) in P493-6 cells with a time course after tetracycline withdrawal (0, 2, 4, 6, 8, 10, 12, 16, 24, 32 and 48hrs after tetracycline removal). MYC was used as a positive control. MAX mRNA level was not found to be affected by MYC induction in P493 cells (not shown).