Expression of Stem Cell Markers in the Human Fetal Kidney
Figure 7
Gene expression analysis in sorted PSA-NCAM subpopulations.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of (a) renal stem/progenitor genes (Six2, Cited1, Sall1, Wt1 and Pax2), (b) vimentin and E-cadherin genes expression in PSA-NCAM magnetically separated cells from HFK (15–19 weeks of gestation). Normalization was performed against control HPRT expression and RQ calculated relative to the PSA-NCAM− fraction. Data were calculated as average±SD of at least 3 independent samples. ***P<0.001, *P<0.05 versus PSA-NCAM−. Sall1 expression in NCAM+ EpCAM+ cells was near significance (p<0.059).