Global Chromatin Architecture Reflects Pluripotency and Lineage Commitment in the Early Mouse Embryo
Figure 5
Chromatin forms compact domains along the nuclear envelope and throughout the nucleoplasm in embryonic and extra-embryonic progenitors at E5.5.
Low magnification fluorescence micrograph (upper left) of E5.5 embryo, showing EPI and extra-embryonic ectoderm (ExE). Low magnification mass-sensitive image of (a) EPI and (b) ExE. Merged phosphorus and nitrogen maps of the indicated fields in (a) and (b) are shown at two magnifications. In both EPI and ExE, chromatin accumulates into compact rim along the nuclear envelope (arrowheads) and in compact blocks at the nuclear envelope and throughout the nucleoplasm (arrows) with little dispersed 10 nm chromatin visible between the compact chromatin domains.