Tumorigenic WAP-T Mouse Mammary Carcinoma Cells: A Model for a Self-Reproducing Homeostatic Cancer Cell System
Figure 11
Repopulation activity of G-2 cell subsets depends on cellular interactions.
(A, B) CD24alow/CD49flow cells were labeled with DiI and 1×104 labeled cells were either cultured separately (A), or plated at a 1∶1 ratio with non-labeled CD24ahigh/CD49fhigh cells (B) in 6-well plates. After 3 days in culture, DiI labeled cells were analyzed for expression of CD24a and CD49f by FACS. (C, D) 1×104 cells of the Epcamlow subset expressing eGFP were either cultured alone (C) or plated at a 1∶1 ratio with G-2 cells lacking eGFP from the Epcamhigh subset (D) in 6-well plates. After 3 days in culture, GFP positive cells were analyzed for Epcam expression by FACS. The representative FACS dot plots are shown.