Targeted Amino-Terminal Acetylation of Recombinant Proteins in E. coli

doi:10.1371/journal.pone.0015801

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Targeted Amino-Terminal Acetylation of Recombinant Proteins in E. coli

Figure 3

Electron-spray mass-spectroscopy spectra for S. pombe Cdc8.

Cdc8 was purified from E. coli BL21-pNatB cells. The un-deconvolved mass charge envelope spectra show the purity of the protein. Deconvolution of this data indicates that all of the Cdc8 purified from BL21-pNatB cells is acetylated.

doi: https://doi.org/10.1371/journal.pone.0015801.g003