Structuring Detergents for Extracting and Stabilizing Functional Membrane Proteins
Figure 4
BmrA purification with C4Cn and detergent exchange.
(A) SDS-PAGE of the sequential extraction of BmrA. As detailed in Methods, the membrane fraction (lane T) was incubated with C4C3 and then centrifuged to give the supernatant S and the pellet P. The latter, enriched in BmrA, was suspended in the presence of C4C7 and then centrifuged to give the corresponding supernatant S and pellet P. Arrows indicate the position of BmrA, C4C3 and C4C7. The C4C7 supernatant was then subjected to DLS (B), Ni-affinity chromatography (C) and gel filtration carried out with FC12 (D) from which respective pools indicated by stars were loaded onto SDS-PAGE.