CCN2 Is Required for the TGF-β Induced Activation of Smad1 - Erk1/2 Signaling Network
Figure 5
CCN2 induces Src and Erk1/2 phosphorylation.
(A) Foreskin fibroblasts were serum starved overnight and then stimulated with indicated doses of recombinant CCN2 (rCCN2) (5 - 100 ng/ml) for 30 minutes and analyzed for Src and Erk1/2 phosphorylation. (B) Foreskin fibroblasts were serum starved overnight and then stimulated with rCCN2 (25 ng/ml) for the indicated time points and analyzed for Src and Erk1/2 phosphorylation. (C) Foreskin fibroblasts were transfected with β3 siRNA oligos and then stimulated with rCCN2 for 30 minutes and examined the Src and Erk1/2 phosphorylation by western blot. (D) Foreskin fibroblasts were treated with rCCN2 for 48 hours and then were analyzed for Smad1 expression levels by western blot.