Over-Expression of PDGFR-β Promotes PDGF-Induced Proliferation, Migration, and Angiogenesis of EPCs through PI3K/Akt Signaling Pathway
Figure 2
PDGFR-β expression and localization in EPCs.
(A)RT- PCR analysis of PDGFR-β (296 bp) in EPCs at Day 4, 7, 14, and 21. (B) Western blot analysis of PDGFR-β protein (124 kDa) in EPCs at Day 4, 7, 14, and 21. (C, D, E) Subcellular localization of PDGFR-β in EPCs. EPCs were stained for PDGFR-β (C) and nuclei were stained with DAPI (D). PDGFR-β was localized predominantly to the plasma membrane of EPCs. PDGFR-β positive cells in the total cells were 88.6±4.3% (n = 5; three random fields per well).Scale bar = 25 µm.