Over-Expression of PDGFR-β Promotes PDGF-Induced Proliferation, Migration, and Angiogenesis of EPCs through PI3K/Akt Signaling Pathway
Figure 8
The role of PI3K/Akt pathway in PDGF-BB-induced proliferation, migration, and tube formation of EPCs.
(A, B, C) Cells in the control group (bright bars, left half) and the pEGFP-N2-PDGFR-β group (dark bars, right half) were without pretreatment or pretreated with AG1295, LY294002, or sc-221226 for 1 h and then treated with 20 ng/mL PDGF-BB (for the control cells) or 80 ng/mL PDGF-BB (for the pEGFP-N2-PDGFR-β cells). PDGF-BB-induced proliferation (A), migration (B), and angiogenesis (C) of EPCs were significantly inhibited by pretreatment with AG1295, LY294002, or sc-221226. ** P<0.01 vs. control cells under 0 ng/mL PDGF-BB stimulation; * P<0.01 vs. control cells under 20 ng/mL PDGF-BB stimulation. ## P<0.01 vs. pEGFP-N2-PDGFR-β cells under 0 ng/mL PDGF-BB stimulation; # P<0.01 vs. pEGFP-N2-PDGFR-β cells under 80 ng/mL PDGF-BB stimulation.