Dvl2-Dependent Activation of Daam1 and RhoA Regulates Wnt5a-Induced Breast Cancer Cell Migration
Figure 1
Wnt5a promotes MDA-MB-231 and MCF-7 cell migration.
(A, B) MDA-MB-231 cells were stimulated by rWnt5a at the indicated doses for 4 h. The cell motility rate was measured by wound healing assays (A) or Boyden chamber assays (B). Magnification, ×100 (A) and ×200 (B). (C) MDA-MB-231 cells were preincubated with Wnt5a inhibitor (sFRP2) for 1 h at the indicated doses, and migration in response to rWnt5a (500 ng/mL, for 4 h) was measured by wound healing assays. (D) MCF-7 cells were preincubated with sFRP2 for 1 h at the indicated doses, and migration was measured by Boyden chamber assays after 48 h. Results are presented as mean ± s.d. of 5 independent experiments in (A) to (D).