Increased Expression of PITX2 Transcription Factor Contributes to Ovarian Cancer Progression
Figure 1
PITX2 is upregulated in ovarian cancer samples and cell lines.
(A) Quantitative RT-PCR analysis was performed in normal ovaries (n = 54) and ovarian cancer samples (n = 97) using PITX2 specific primer. 18S and TATA-box binding protein (TBP) were used as the internal loading controls. *P<0.001. (B) Western blot analysis using anti-PITX2 antibody to evaluate the expression level of PITX2 (isoforms A, B and C)) (35 kDa) in ovarian cancer cell lines (n = 9) and HOSE cell lines (n = 2). β-actin was used as a loading control. (C) Immunohistochemical analysis of PITX2 expression (nuclear staining) in borderline cystadenoma and high-grade (3) serous cystadenocarcinoma on an ovarian cancer tissue array (OVC1021). Magnification: 20×.