The bHLH Repressor Deadpan Regulates the Self-renewal and Specification of Drosophila Larval Neural Stem Cells Independently of Notch
Figure 4
Pros mediates premature terminal division of dpn mutant NBs.
(A–A’’) Wild type NBs (large arrows) do not accumulate nuclear Pros at the 3rd instar larval stage. Instead, only cortical Pros is detected (the small arrow in (A’)). (B–B’’) Nuclear Pros is detected in a dpn mutant type I NB (arrowhead) at the 3rd instar larval stage, while a neighboring NB (arrow) remains negative for nuclear Pros. NBs in (A–B) are labeled with Mira staining in green. (C–E) Wild type (C), prosv17 (D), and dpn prosv17 double mutant (E) type I NB clones at the 3rd instar larval stage. Clones were labeled with mCD8-GFP and stained for Ase in red. Arrows point to NBs in the clones. (F) The number of dpn mutant type I NBs at late 3rd instar larval brains is partially rescued in prosv17 heterozygous mutant background, whereas the number of type I NBs in prosv17 heterozygous larval brains is similar to that in wild type brains.