SDG2-Mediated H3K4 Methylation Is Required for Proper Arabidopsis Root Growth and Development
Figure 5
Loss of SDG2 synergistically enhances growth defects of the CAF1 loss-of-function mutant fas2-4. A
, Representative example of 14-day-old seedling of the wild-type Col, the single mutants sdg2-3 and fas2-4, and the double mutant sdg2-3 fas2-4. Bar = 1 cm. B, Comparison of primary root length between fas2-4 and sdg2-3 fas2-4 on 16-day-old seedlings. Root length is shown as a mean value from two independent experiments with each comprising at least 20 plants. Bar indicates SD. C and D, Comparison of root cap cell organization between fas2-4 and sdg2-3 fas2-4, respectively. DIC images were taken on Lugol-stained root tips of 6-day-old seedlings. Arrowhead in C indicates QC position. Bar = 50 µm. E and F, Comparison of cell layer organizations of root apical meristem between fas2-4 and sdg2-3 fas2-4, respectively. Confocal images were taken on PI-stained roots of 6-day-old seedlings. The QC cell in E is marked in blue. Bar = 50 µm.