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Genistein Inhibits Prostate Cancer Cell Growth by Targeting miR-34a and Oncogenic HOTAIR

Figure 3

miR-34a regulates HOTAIR expression.

(A) Putative miR-34a binding and mutated sites in HOTAIR. (B) Luciferase reporter assays using vectors encoding putative binding sites. PC3 and DU145 cells were transiently transfected with Pre-miR miRNA precursor or negative control, followed by transient transfection with basic vector or wild-type reporter plasmids or mutated plasmids for 24 hours. Reporter activity was measured by luciferase assay and normalized to the activity of Renilla luciferase. Data are presented as the mean ± SE. *, P<0.05. (C) The expression level of HOTAIR was determined by quantitative real-time PCR analyses after transfection with miR-34a mimics and negative control in PCa cell lines (PC3 and DU145). HOTAIR expression was normalized to GAPDH. *, P<0.05.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0070372.g003