Intrinsic Levanase Activity of Bacillus subtilis 168 Levansucrase (SacB)
Fig 5
Evolution of low molecular weight levan during hydrolysis by SacB (500 nM) as determined by GPC.
The reaction took place at 25°C with LMw levan (0.85 mM) in a total reaction volume of 1.4 mL in the working buffer. These are the same concentrations employed in the ITC experiment depicted in Figs 3 and 4.