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Selecting Superior De Novo Transcriptome Assemblies: Lessons Learned by Leveraging the Best Plant Genome

Fig 1

Illumina Sequence coverage of Arabidopsis cDNAs.

Coverage of all detected genes by sequencing reads. The darkest bar represents the number of detected genes not tagged, and each progressively lighter bar represents genes in a bin with a 5% increase in coverage, with the two lightest bars showing the number of genes covered at >90% and >99%, respectively. Normalized BR12 = Normalized Illumina library from pooled biological replicates 1 and 2, BR12 = combined coverage of Illumina biological replicates 1 and 2, BR1 = Illumina biological replicate 1, BR2 = Illumina biological replicate 2.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0146062.g001