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A morphological method for ammonia detection in liver

Fig 3

Hepatic ammonia staining in mouse models of Non-Alcoholic Fatty Liver Disease (NAFLD).

Representative micrographs of Sudan Red, Sirius Red, F4/80 and ammonia staining in liver samples from high-fat (HF) diet (n = 6 Ctrl vs. n = 7 HF diet), (A) and choline deficient with 0.1% methionine diet (MCDD)-fed rodents (n = 6 Ctrl vs. n = 5 MCDD 2 weeks and n = 5 MCDD 6 weeks) (B). AS was only significantly increased in MCDD 6 weeks vs. Ctrl, p<0.05.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0173914.g003