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Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages

Fig 3

Phagocytosis assay.

Results of phagocytosis assay performed on RAW 264.7 cells at various passages. Graphs A, C, E show percentage of phagocyting cells, positive for FITC fluorochrome conjugated with latex beads. Graphs B, D, F show the intensity of phagocytosis expressed as mean fluorescence intensity of latex beads. A, B–phagocytosis of RAW 264.7 cells. C, D–phagocytosis of LPS treated RAW 264.7 cells. E, F–comparison of phagocytosis ability of RAW 264.7 cells in various condition, ice control indicating technical control for phagocytosis assay. *- p-value < 0.05, **—p-value < 0.01.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0198943.g003