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Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages

Fig 4

Nitrogen level in cell culture medium.

Level of nitrogen in cell culture medium of RAW 264.7 cells at various passages. A—comparison of NO level in control cells and LPS stimulated cells. B– 24 hrs production of nitric oxide by LPS stimulated RAW 264.7 cells. Data presented as average concentration of produced nitrites, calculated on the standard curve basis. *—p-value < 0.05, **—p-value < 0.01, ***—p-value < 0.001, ****—p-value < 0.0001.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0198943.g004