Understanding leptospirosis eco-epidemiology by environmental DNA metabarcoding of irrigation water from two agro-ecological regions of Sri Lanka
Fig 3
Molecular phylogenetic tree of leptospiral flaB genes.
In total 432 nucleotide sites of flaB genes determined from environmental DNA analysis of the present study (shown in gray shading) were aligned and analyzed against known flaB sequences of representative Leptospira species. The GenBank accession numbers of the reference sequences were shown within sequence names. Maximum-likelihood phylogenetic analysis was performed with GTR + G + I model of nucleotide substitution. Numbers on the tree indicate support values for the nodes estimated from 100 bootstrap replications. K03 denotes the sample name collected from Kandy.