BIOTECTNOLOGY AND ITS APPLICATIONS.
NEET QUESTIONS
1. Insect resistant transgenic cotton has been produced by inserting a piece of DNA from
a. An insect b. A bacterium
c. A wild relative of cotton c. A virus
2. Hybridoma technology has been successfully used in
a. Production of somatic hybrids b. Synthesis of monoclonal
antibiotics
c. Synthesis of haemoglobin d.Pproduction of alcohol in bulk
3. D
a. Vaccine b. Plasmid
c. Bacterium d. Protozoa
4. Name the drug used in cancer treatment produced by using biotechnology
a. Terramycin b. HGH
c. . Interferon d. TSH
5. Cultivation of Bt cotton has been much in the news. The prefix Bt means
a.
b.
c. Produced by
d. Carrying an endotoxin gene from Bacillus thrungienesis
6. T
a. Louis Pasteur b. R.Koch
c. W.Fleming d. A.Fleming
7. In transgenics, expression of transgene in target tissue is determined by
a. Transgene b. Promoter
c. Reporter d. Enhancer
8. Terminator gene
a. Help in terminating flowering b. Help in terminating seed
germination
c. Used in hybridization d. None of these
9. First biochemical to be produced commercially by microbial cloning and genetic engineering
a. Human insulin b. Penicillin
c. Interferons d. Fertility factor
10. Golden rice is a transgenic crop of the future with the following improved trail
a. Insect resistance b. High lysine content
c. High protein content d. High vitamin-A content
11. Bacillus thuringiensis (Bt) strains have been used for designing novel
a. Biofertilizers b. Bio-metaluurgical techniques
c. Bio-mineralization process d. Bio-insecticidal plants
12. Which of the following is a correct statement
a. -
through biotechnology
b. Somatic hybridization involves fusion of two complete plant cells carrying desired
genes
c. The anticoagulant hirudin is being produced from transgenic Brassica napus seed
d.
improves its tastes its taste
13. Which bacteria is used as biopesticide first on the commercial scale in the world?
a. Bacillus thuringiensis b. E.coli
c. Pseudomonas aeruginosa d. Agrobacterium tumefaciens
14. Gene recombinant technology is used for
a. Vector less gene transfer into target cell
b. Vector based gene transfer into target cell
c. Direct transfer of DNA protein complex
d. Liposome base direct gene transfer into target cell
15. Transfer of DNA bands from an agrose gel to a nitrocellulose or nylon membrane is referred
to as
a. Western transfer b. Northern transfer
c. Eastern transfer d. Gene transfer
e. Southern transfer
16. Golden rice is a promising transgenic crop. When released for cultivation, it will help in
a. Producing a petrol-like fuel from rice b. Alleviation of vitamin A
c. Pest resistance d. Herbicide tolerance
17. A genetically engineered micro-organism used successfully in bioremediation of oil spills is a
species of
a. Trichoderma b. Xanthomonas
c. Bacillus d. Pseudomonas
18. Blindness is prevented by use of which crop in poor countries?
a. Golden rice b. Wheat
c. Gram d. Pea
19. Human insulin is being commercially produced from a transgenic species of
a. Rhizobium b. Saccharomyces
c. Escherichia d. mycobacterium
20. A transgenic food crop which may help in solving the problem of night blindness in
developing countries is
a. Bt soybean b. Golden rice
c. Flavrsavrtomatoes d. Starlink maize
21. Main objective of production/use of herbicide resistant GM crops is to
a. Encourage eco-friendly herbicides
b. Reduce herbicide accumulation in food articles for healt h safety
c. Eliminate weeds from the field without the use of manual labour
d. Eliminate weeds from the field without the use of herbicide
22. Genetically engineered bacteria are being employed for production of
a. Thyroxine b. Human insulin
c. Cortisol d. Epinephrine
23. Isolation of Bt gene from bacterium (Bacillus thuringiensis) was taken up in the year
a. 1977 b. 1980
c. 1997 d. 1990
24. Which one of the following is commonly used in transfer of foreign DNA into crop plants?
a. Meloidogyne incognita b. Agrobacterium tumefaciens
c. Pencillium expansum d. Trichoderma harzianum
25. What is true about Bt toxin?
a. Bt toxin exists as active toxin in the Bacillius.
b. The activated toxin enters the ovaries of the pest to sterlise it and thus prevents its
multiplication.
c. The concerned Bacillus has anti toxin
d. The inactive prototoxin gets converted into active form in the insect gut
26. Transgenic plants are the ones
a. Generated by introducing foreign DNA into a cell and regenerating a plant from the
cell
b. Produced after protoplast fusion in artificial medium
c. Grown in artificial medium after hybridization in the field.
d. Produced by a somatic embryo in artificial medium
27. The bacteria Bacillus thuringiensis is widely used in contemporary biology as
a. Insecticide b. Agent for production of diary
products
c. Source of industrial enzyme d. Indicator of water pollution
28. Golden rice is
a. A variety of rice grown along the yellow river in china
b. Long stored rice having yellow colour tint
c. A -carotene
d. Wild variety of rice with yellow coloured grains
29. In RNAi,genes are silenced using
a. ss DNA b. ds DNA
c. ds RNA e. ss RNA
30. The first clinical gene therapy was done for the treatment of
a. AIDS b. Cancer
c. Cystic fibrosis d. SCID
31. ADA is an enzyme which is deficient in a genetic disorder SCID. What is the full form of ADA?
a. Adenosine deoxyaminase b. Adenosine deaminase
c. Aspartate deaminase d. Arginine deaminase
32. Silencing of a gene could be achieved through the use of
a. Short interfering RNA(RNAi) b. Antisense RNA
c. By both c. None of the above
33. Silencing of m-RNA has been used in producing transgenic plants resistant to
a. Bollworms b. Nematodes
c. White rusts d. Bacterial blights
34. The first clinical gene therapy was given for treating
a. Diabetes mellitus b. Chicken pox
c. Rheumatoid arthritis d. Adenosine deaminase deficiency
35. Tobacco plants resistant to a nematode have been developed by the introduction of DNA
that produces(in the host cell)
a. Both sense and anti-sense RNA b. A particular hormone
c. An antifeedant d. A toxin protein
36. Amplification of gene of interest by using DNA polymerase may go upto
a. 0.1 million times b. 1.0 million times
c. 1.0 billion times d. 1.0 trillion times
37. Which of the following Bt crops is being grown in India by the farmers?
a. Cotton b. Brinjal
c. Soybean d. Maize
38. The first human hormone produced by recombinant DNA technology is
a. Insulin b. Estrogen
c. Thyroxin d. Progesterone
39. An analysis of chromosomal DNA using the hybridization technique does not use
a. Electrophoresis b. Blotting
c. Autoradiography d. PCR
40. Bt cotton is not:
a. A GM plant
b. Insect resistant
c. A bacterial gene expressing system
d. Resistant to all pesticides
41. C-peptide of human insulin is:
a. A part of mature insulin molecule
b. Responsible for formation of disulphide bridges
c Removed during maturation of pro-insulin to insulind. Responsible for its biological activity.
42. GEAC stands for:
a. Genome Engineering Action Committee
b. Ground Environment Action Committee
c. Genetic Engineering Approval Committee
d. Genetic and Environment Approval committee
-1 antitrypsin is:
a. An antacid
b. An enzyme
c. Used to treat arthritis
d. Used to treat emphysema
44. The site of production of ADA in the body is:
a. Bone marrow
b. Lymphocytes
c. Blood plasma
d. Monocytes
45.A protoxin is:
a. A primitive toxin
b. A denatured toxin
c. Toxin produced by protozoa
d. Inactive toxin
46. The trigger for activation of toxin of Bacillus thuringiensis is:
a. Acidic pH of stomach
b. High temperature
c. Alkaline pH of gut
d. Mechanical action in the insect gut
47. In RNAi, genes are silenced using:
a. ss DNA
b. ds DNA
c. ds RNA
d. ss RNA
48.
a. Restriction endonuclease
b. DNA polymerase
c. DNA ligase
d. Helicase
49. Plants are genetically engineered with novel genes by
a.Embryo rescue technique
b. Recombination breeding
c. Protoplast fusion
d. Recombinant DNA technology
50. Maximum application of animal culture technology today is in the production
a) Insulin
b) Interferons
c) Edible proteins
d) Vaccines.
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