OM COACHING CLASSES
Class : XIIth Subject : BIOLOGY
Date : Solutions DPP No. : 2
Topic :- Biotechnology Principles & Processes
1. Common bacterium used in genetic engineering is:
a) 𝐸. 𝑐𝑜𝑙𝑖 b) 𝐷𝑖𝑝𝑙𝑜𝑐𝑜𝑐𝑐𝑢𝑠 c) 𝑅ℎ𝑖𝑧𝑜𝑏𝑖𝑢𝑚 d) 𝑆𝑝𝑖𝑟𝑖𝑙𝑙𝑖𝑢𝑚
2. Who discovered that restriction enzymes have the capability of cutting DNA strands in a particular
fashion, which left what has became known as ‘sticky ends’ on the strands?
a) Ramdeo Mishra b) Stanley Cohen c) Herbert Boyer d) James D Watson
3. A restriction fragment containing a specific gene of interest can be identified by gel electrophoresis
followed by transferring the DNA to a membrane as a solid support matrix using a procedure called
a) An allozyme b) A southern blot
c) Identification of a gene d) A restriction fragment length polymorphism
4. About gene gun method
I. This method is also known as biolistic technique
II. In this method cells are bombarded with high velocity micro-particles of gold or tungsten coated with
DNA in plants
III. Important crop plants like maize, rice and wheat have now been transformed by this method
Which of the statements given above are correct?
a) I and II b) I and III c) II and III d) I, II and III
5. Identify the correct match for the given diagram
a) Electrophoresis – Migration of undigested and digested set of DNA fragments
b) Bioreactor – Raw materials are biologically converted into specific products
c) Microinjection – Technique of introducing foreign genes into a host cell
d) Gene cloning – Technique of obtaining identical copies of a particular DNA segment
6. In DNA fingerprinting which of the following is true?
a) VNTR is used as probes
b) Specific metabolic genes are used as probes
c) House keeping or luxury genes are use as probes
d) All of the above
7. The message from nuclear DNA for the synthesis of specific cytoplasmic protein is carried by:
a) mRNA b) t-RNA c) s-RNA d) r-RNA
8. The recent techniques used for separating fragments of DNA is:
a) Northern blotting b) Southern blotting c) Eastern blotting d) Western blotting
9. The flowchart given below represent the process of recombinant technology. Identify 𝐴 and 𝐷
a) A-Restriction endonuclease, B-Restriction exonuclease, C-RNA ligase, D-Transformation
b) A-Restriction endonuclease, B-Restriction endonuclease, C-DNA ligase, D-Transformation
c) A-Restriction exonuclease, B-Restriction endonuclease, C-DNA polymerase , D-Transduction
d) A-Restriction endonuclease, B-Restriction endonuclease, C-DNA polymerase, D-Transformation
10. RNA is removed by the treatment with
a) Ribonuclease b) Protease c) Chitinase d) Cellulase
11. Which one of the following scientists developed the process of DNA fingerprinting?
a) Kary B. Mullis b) T.H. Morgan c) H.O. Smith d) Alec Jeffreys
12. Which of the following statement is not correct regarding 𝐸𝑐𝑜 RI restriction endonuclease enzyme?
I. 𝐸𝑐𝑜. RI restriction endonuclease enzyme is isolated from 𝐸𝑠𝑐ℎ𝑒𝑟𝑖𝑐ℎ𝑖𝑎 𝑐𝑜𝑙𝑖 RY13
II. Its recognition sequence is 5’–GAATTC – 3’
3’–CTTAAG – 5’
↓
5’ – G – A – A –T – T – C – 3’
III. Its site of cleavage is
3’ – C – T – T – A – A – G – 5’
↑
a) I and II
b) I and III
c) I, II and III
d) None of the above
13. Process of formation of RNA from DNA is called
a) Transduction b) Transcription c) Transformation d) Translation
14. Which of the following would not be used in preparing recombinant DNA?
a) Plasmids b) Phages
c) Restriction enzymes d) DNA polymerase III
15. Which one of the following bacteria has found extensive use in genetic engineering work in plants?
a) 𝐴g𝑟𝑜𝑏𝑎𝑐𝑡𝑒𝑟𝑖𝑢𝑚 𝑡𝑢𝑚𝑒𝑓𝑎𝑐𝑖𝑒𝑛𝑠 b) 𝐶𝑙𝑜𝑠𝑡𝑟𝑖𝑑𝑖𝑢𝑚 𝑠𝑒𝑝𝑡𝑖𝑐𝑢𝑚
c) 𝑋𝑎𝑛𝑡ℎ𝑜𝑚𝑜𝑛𝑎𝑠 𝑐𝑖𝑡𝑟𝑖 d) 𝐵𝑎𝑐𝑖𝑙𝑙𝑢𝑠 𝑐𝑜𝑎g𝑢𝑙𝑒𝑛𝑠
16. Which of the following components are used in gel electrophoresis?
I. Ethidium bromide
II. Restriction endonuclease
III. Agarose
IV. UV radiation
Choose the correct option
a) I and II b) I and III c) I, II and IV d) I, II, III and IV
17. What is the first step in Southern Blotting technique?
a) Isolation of DNA from a nucleated cell such as the one from the scene of crime
b) Denaturation of DNA on the gel for hybridization with specific probe
c) Production of group of genetically identical cells
d) Digestion of DNA by restriction enzyme
18. The most thoroughly studied of the known bacteria-plant interaction is the:
a) Plant growth simulation by phosphate-solubilising bacteria
b) Cyanobacterial symbiosis with some aquatic ferns
c) Gall formation on certain angiosperms by Agrobacterium
d) Nodulation of Sesbania stems by nitrogen fixing bacteria
19. Microorganisms can be grown in the bioreactor by
a) Support growth system b) Agitated growth system
c) Suspended growth system d) Both (a) and (b)
20. In Northern blotting RNAs are separated by gel electrophoresis and the RNA bands are transferred onto a
membrane of:
a) Diazobenzyl oxymethyl b) Diazobenzene
c) Diazobromine d) None of the above
