OM COACHING CLASSES
Class : XIIth Subject : BIOLOGY
Date : Solutions DPP No. : 3
Topic :- Biotechnology Principles & Processes
1. Which one of the following is commonly used in transfer of foreign DNA into crop plants?
a) Trichoderma harzianum b) Meloidogyne incognitia
c) Agrobacterium tumefaciens d) Penicillium expansum
2. Which one among the following is just a cloning plasmid not an expression plasmid?
a) pBAD-18-Cam b) pBCSK c) pUC 18 d) pET
3. There …A… are the DNA molecules that can carry a foreign …B… segment into the host cell.
Here A and B refers to
A B
a) Vector RNA b) Vector DNA
c) Gene RNA d) Gene DNA
4. Probes, used in DNA fingerprinting are initially
a) Single-stranded RNA b) Mini satellite
c) 19 base long oligonucleotides d) All of the above
5. Application of PCR are
I. detection of pathogens
II. diagnosis of specific mutation
III. DNA fingerprinting
Choose the correct option
a) I and II b) I and III c) II and III d) I, II and III
6. A clone of sheep Dolly has been made by:
a) Gene transfer b) Somatic cell cloning
c) Nucleus transfer d) Germinal cell cloning
7. T1 -plasmid used in genetic engineering is obtained from
a) Bacillus thuringiensis b) Agrobacterium rhizogenes
c) Agrobacterium tumefaciens d) Psedomonas syringae
8. The role of DNA ligase in the construction of a recombinant DNA molecule is
a) Formation of phosphodiester bond between two DNA fragments
b) Formation of hydrogen bonds between sticky ends of DNA fragments
c) Ligation of all purine and pyrimidine bases
d) None of the above
9. Transgenic animals are produced by injecting foreign gene into the:
a) Egg b) Nucleus of unfertilized egg
c) Nucleus of fertilized egg d) Nucleus of sperm
10. Clonal cell lines can be obtained by:
a) Autoradiography b) Tissue culture c) Centrifugation d) Cell fractionation
11. Electroporation procedure involves:
a) Fast passage of food through sieve pores in phloem elements with the help of electric stimulation
b) Opening of stomatal pores during night by artificial light
c) Making transient pores in the cell membrane to introduce gene constructs
d) Purification of saline water with the help of a membrane system
12. Which of the following is associated with genetic engineering?
a) Plastids b) Plasmids c) Mutations d) Hybrid vigour
13. Biolistics (gene gun) is suitable for
a) Disarming pathogen vectors b) Transformation of plants cells
c) Construction recombinant DNA by joining with d) DNA fingerprinting
vectors
14. Which of the following statements are correct for the enzyme 𝑡𝑎𝑞 polymerases?
I. 𝑇𝑎𝑞 polymerase is thermally unstable
II. It requires primers for carrying out the process of polymerization
III. 𝑇𝑎𝑞 polymerase is isolated from thermophilic bacterium, 𝑇ℎ𝑒𝑟𝑚𝑢𝑠 𝑎𝑞𝑢𝑎𝑡𝑖𝑐𝑢𝑠
Choose the correct option
a) I and II b) I and III c) II and III d) I, II and III
15. EFB stands for
a) European Federation of Biotechnology b) Eurasian Federation of Biotechnology
c) East Asia Federation of Biotechnology d) Ethiopian Federation of Biotechnology
16. The commonly used DNA fingerprinting technique in forensic studies is simply a method involving
a) Southern blotting b) Northern blotting c) Eastern blotting d) Western blotting
17. 𝐶𝑟𝑦 I endotoxins obtained from 𝐵𝑎𝑐𝑖𝑙𝑙𝑢𝑠 𝑡ℎ𝑟𝑢𝑖𝑔𝑖𝑒𝑛𝑠𝑖𝑠 are effective against
a) Nematodes b) Bollworms c) Mosquitoes d) Flies
18. In the naming of restriction enzymes the first letter is derived from …A… name and next two letters from
the …B… and fourth letter from …C… of …D… where the enzymes are extracted
A to D in the statement can be
A B C D
a) Genus species strain bacteria b) Species genus strain bacteria
c) Genus species variety eukaryote d) Species genus variety eukaryote
19. Which of the following techniques is most commonly used to separate DNA molecules by size?
a) Chromatography b) PCR c) RFLP d) Gel electrophoresis
20. Which one of the following scientists got the Nobel Prize for his invention polymerase chain reaction
(PCR)?
a) F. Sanger b) Paul Berg c) Alec Jeffreys d) Kary B. Mullis
