BP701T:INSTRUMENTAL METHODS OF ANALYSIS
L:3 T:1 P:0 Credits:4
Course Outcomes: Through this course students should be able to
CO1 :: demonstrate the principle, instrumentation and applications of spectroscopy techniques
CO2 :: interpret the spectra of compounds in IR spectroscopy
CO3 :: compare the principle and applications of flame emission and atomic absorption
spectroscopy
Unit I
UV Visible spectroscopy : Electronic transitions, Chromophores, Auxochromes, Spectral shifts,
Solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and Deviations.,
Instrumentation - Sources of radiation, Wavelength selectors, Sample cells, Detectors- Photo tube,
Photomultiplier tube, Photo voltaic cell, Silicon Photodiode, Applications - Spectrophotometric
titrations, Single component and multi component analysis.
Fluorimetry : : Theory, Concepts of singlet, doublet and triplet electronic states, Internal and
External conversions, Factors affecting fluorescence, Quenching, Instrumentation and applications.
Unit II
IR spectroscopy : : Introduction, fundamental modes of vibrations in poly atomic molecules, sample
handling, factors affecting vibrations., Instrumentation - sources of radiation, wavelength selectors,
detectors - golay cell, bolometer, thermocouple, thermister, pyroelectric detector and applications.
Flame photometry : : Principle, interferences, instrumentation and applications.
Unit III
Atomic absorption spectroscopy : :Principle, interferences, instrumentation and applications.
Nepheloturbidometry : : Principle and instrumentation, applications.
Unit IV
Introduction to chromatography:
Adsorption and partition column chromatography: : : Methodology, advantages and
disadvantages, applications.
Electrophoresis : : Introduction, factors affecting electrophoretic mobility, techniques of paper, gel,
capillary electrophoresis, applications.
Paper chromatography : : Introduction, Methodology, development techniques, advantages,
disadvantages and applications.
Thin layer chromatography : : Introduction, principle, methodology, Rf values, advantages,
disadvantages and applications.
Unit V
High performance liquid chromatography (HPLC) : : introduction, theory, instrumentation,
advantages and applications.
Gas Chromatography : : Introduction, theory, instrumentation, derivatization, temperature
programming, advantages, disadvantages and applications.
Unit VI
Ion exchange chromatography : : Introduction, classification, ion exchange resins, properties,
mechanism of ion exchange process, factors affecting ion exchange, methodology and applications.
Gel chromatography : : Introduction, theory, instrumentation and applications.
Affinity chromatography : Introduction, theory, instrumentation and applications.
Text Books:
1. INSTRUMENTAL METHODS OF CHEMICAL ANALYSIS by CHATWAL, G.R., ANAND, S.K.,,
HIMALAYA PUBLISHING HOUSE PVT. LTD
References:
1. TEXTBOOK OF PHARMACEUTICAL ANALYSIS by KENNETH A. CONNORS, WILEY
2. ELEMENTARY ORGANIC SPECTROSCOPY by SHARMA Y.R, S Chand Publishing
3. ORGANIC SPECTROSCOPY by WILLIAM KEMP, MACMILLAN
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