SVKM’s Narsee Monjee Institute of Management Studies
Pharmacy & Technology Management
Program: B. Pharm Semester : VII
Course : Instrumental Methods of Analysis - Theory Code:BP701T
Teaching Scheme Evaluation Scheme
Lecture Practical Tutorial Credit Term End
Internal Continuous
(Hours (Hours Examinations (TEE)
Assessment (ICA)
per per (Marks- 75 in
(Marks - 25)
week) week) Question Paper)
3 NA 1 4 Marks Scaled to 25 Marks Scaled to 75
Prerequisite : Basic knowledge of chemistry and spectroscopic techniques
Scope: This subject deals with the application of instrumental methods in qualitative and
quantitative analysis of drugs. This subject is designed to impart a fundamental knowledge on
the principles and instrumentation of spectroscopic and chromatographic technique. This also
emphasizes on theoretical and practical knowledge on modern analytical instruments that are
used for drug testing.
Objectives: The course is designed to understand separation techniques (Thin layer
chromatography, paper chromatography, column chromatography, gas chromatography, high
pressure liquid chromatography), characterization techniques (NMR, Mass spectroscopy),
modern hyphenated techniques (LC-MS, LC-MS/MS, GC-MS) and analytical method
validation.
Outcomes: Upon completion of the course the student shall be able to:
• Understand the interaction of matter with electromagnetic radiations and its applications in
drug analysis
• Understand the chromatographic separation and analysis of drugs.
• Perform quantitative & qualitative analysis of drugs using various sophisticated analytical
instruments.
Detailed Syllabus: ( per session plan )
Unit Description Duration
1 UV Visible spectroscopy 10
Electronic transitions, chromophores, auxochromes, spectral shifts,
solvent effect on absorption spectra, Beer and Lambert’s law, Derivation
and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample
cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell,
Silicon Photodiode.
Applications - Spectrophotometric titrations, Single component and
multi component analysis
Fluorimetry
Theory, Concepts of singlet, doublet and triplet electronic states, internal
and external conversions, factors affecting fluorescence, quenching,
instrumentation and applications.
Fluorometric Assay of any one Pharmaceutical Drug from IP.
2 IR spectroscopy 10
Introduction, fundamental modes of vibrations in poly atomic molecules,
sample handling, factors affecting vibrations
Instrumentation - Sources of radiation, wavelength selectors, detectors -
Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector
and applications. Principle & pharmaceutical applications of FT-NIR.
Flame Photometry-Principle, interferences, instrumentation and
applications
Atomic absorption spectroscopy- Principle, interferences,
instrumentation and applications
Nepheloturbidometry- Principle, instrumentation and applications
3 Introduction to chromatography 10
Adsorption and partition column chromatography-Methodology,
advantages, disadvantages and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf
values, advantages, disadvantages and applications. Advantages of
HPTLC over TLC. Steps involved in HPTLC Analysis.
Paper chromatography-Introduction, methodology, development
techniques, advantages, disadvantages and applications
Electrophoresis– Introduction, factors affecting electrophoretic mobility,
Techniques of paper, gel, capillary electrophoresis, applications
4 Gas chromatography-Introduction, theory, instrumentation, 8
derivatization, temperature programming, advantages, disadvantages and
applications
High performance liquid chromatography (HPLC)-Introduction, theory,
instrumentation, advantages and applications. Advantages of UPLC over
HPLC
5 Ion exchange chromatography- Introduction, classification, ion exchange 7
resins, properties, mechanism of ion exchange process, factors affecting ion
exchange, methodology and applications
Gel chromatography- Introduction, theory, instrumentation and applications
Affinity chromatography- Introduction, theory, instrumentation and
applications
6 Tutorials 15
Total 60
Text Books:
1. Text book of Pharmaceutical Analysis by Kenneth A. Connors
2. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
3. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
4. Organic Chemistry by I. L. Finar
5. Organic spectroscopy by William Kemp
6. Quantitative Analysis of Drugs by D. C. Garrett
7. Spectrophotometric identification of Organic Compounds by Silverstein
Reference Books:
1. Indian Pharmacopoeia 2014, Volume I, II, III & IV; Government of India, Ministry of Health
and Family Welfare; The Indian Pharmacopoeia Commission, Ghaziabad.
2. National Formulary of India, 2011; Government of India, Ministry of Health and Family
Welfare; Indian Pharmacopoeia Commission.
3. British Pharmacopoeia 2014, Volume 1, 2, 3, 4 & 5; British Pharmacopoeia Commission.
4. USP 37/ NF 32, 2014, Volume 1, 2, 3 & 4; The United States Pharmacopeial Convention,
Rockville, MD.
5. European Pharmacopoeia 2014 8.0; Volume 1 & 2; EDQM Publication.
6. The International Pharmacopoeia; 3rd edition; 1995 Volume 1, 2, 3 & 4; WHO, CBS
Publishers & Distributors.
Any other information :
• http://www.cis.rit.edu/htbooks/nmr/
• http://en.wikipedia.org/wiki/NMR_spectroscopy
• www.astbury.leeds.ac.uk/facil/MStut/mstutorial.htm
• http://teaching.shu.ac.uk/hwb/chemistry/tutorials/chrom/chrom1.htm
• http://en.wikipedia.org/wiki/Chromatography
• http://www.rpi.edu/dept/chem-eng/Biotech-Environ/CHROMO/chromintro.html
• http://www.ptfarm.pl/pub/File/Acta_Poloniae/2012/3/371.pdf
Total Marks of Internal Continuous Assessment (ICA) : 25 Marks
Distribution of ICA Marks :
Description of ICA Marks
Sessional Examination 15
Term Work 10
Total Marks : 25
____________ ____________
Signature Signature
(Prepared by) (Approved by)
Dr. Mayur Yergeri Dr. Bala Prabhakar
Dean, SPPSPTM
