BP701T.
INSTRUMENTAL METHODS OF ANALYSIS (Theory)
45 Hours
Course Content:
Unit -I 10 Hours
UV Visible spectroscopy: Electronic transitions, chromophores, auxochromes, spectral shifts,
solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube,
Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
Applications- Spectrophotometric titrations, Single component and multi component analysis.
Fluorimetry: Theory, Concepts of singlet, doublet and triplet electronic states, internal and
external conversions, factors affecting fluorescence, quenching, instrumentation and
applications.
Unit-II 10 Hours
IR spectroscopy: Introduction, fundamental modes of vibrations in poly atomic molecules,
sample handling, factors affecting vibrations.
Instrumentation- Sources of radiation, wavelength selectors, detectors - Golay cell,
Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.
Flame Photometry- Principle, interferences, instrumentation and applications.
Atomic absorption spectroscopy- Principle, interferences, instrumentation and applications.
Nephelo-turbidimetry- Principle, instrumentation and applications.
Unit-III 10 Hours
Introduction to chromatography:
Adsorption and partition column chromatography- Methodology, advantages, disadvantages
and applications.
Thin layer chromatography- Introduction, Principle, Methodology, Rf values, advantages,
disadvantages and applications.
Paper chromatography- Introduction, methodology, development techniques, advantages,
disadvantages and applications.
Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques of paper,
gel, capillary electrophoresis, applications.
Unit-IV 08 Hours
Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature
programming, advantages, disadvantages and applications.
High performance liquid chromatography (HPLC)- Introduction, theory, instrumentation,
advantages and applications.
Unit-V 07 Hours
Ion exchange chromatography- Introduction, classification, ion exchange resins, properties,
mechanism of ion exchange process, factors affecting ion exchange, methodology and
applications.
Gel chromatography- Introduction, theory, instrumentation and applications.
Affinity chromatography- Introduction, theory, instrumentation and applications.
Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 113
BP705P. INSTRUMENTAL METHODS OF ANALYSIS / NDDS (Practical)
4 Hours/Week
1. Determination of absorption maxima and effect of solvents on absorption maxima of
organic compounds.
2. Estimation of sulphanilamide by colorimetry.
3. Simultaneous estimation of ibuprofen and Paracetamol by UV spectroscopy.
4. Estimation of quinine sulphate by fluorimetry.
5. Study of quenching of fluorescence.
6. Determination of sodium by flame photometry.
7. Determination of potassium by flame photometry.
8. Determination of chlorides and sulphates by nephelo-turbidimetry.
9. Separation of sugars by thin layer chromatography.
10. Separation of plant pigments by column chromatography.
11. Demonstration experiment on HPLC.
12. Demonstration experiment on Gas Chromatography.
13. To perform in-vitro dissolution profile of CR/SR marketed formulation.
14. To prepare sustained release matrix tablets and evaluate by UV spectroscopy.
15. Formulation of nanoparticles and evaluate by HPLC.
16. Formulation and evaluation of liposomes.
17. To prepare buccal dosage form and evaluate by UV spectroscopy.
18. To prepare Paracetamol transdermal patch and evaluate by UV spectroscopy.
Recommended Books (Latest Editions)
• Instrumental Methods of Chemical Analysis by B.K. Sharma, Krishna Prakashan Media
(P) Ltd., Meerut, India.
• Organic Spectroscopy by Y.R Sharma, S. Chand & Company Ltd., New Delhi.
• Pharmaceutical Chemistry Instrumental Technique by Leslie G. Chatten, CBS Publisher
and Distributer Pvt. Ltd., New Delhi.
• Textbook of Pharmaceutical Analysis by Kenneth A. Connors, John Wiley & Sons, Inc.,
New York.
• Vogel’s Textbook of Quantitative Chemical Analysis by A.I. Vogel, Addison Wesley
Logman, Singapore.
• Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake, CBS Publishers &
Distributers Pvt. Ltd., New Delhi.
• Organic Spectroscopy by William Kemp, Palgrave, NY.
• Quantitative Analysis of Drugs by D.C.Garrett, Chapman & Hall Ltd., London.
• Quantitative Analysis of Drugs in Pharmaceutical Formulations by P.D. Sethi, CBS
Publishers & Distributers Pvt. Ltd., New Delhi.
• Spectrophotometric Identification of Organic Compounds by Silverstein, John Wiley &
Sons, Inc., New York.
• Controlled and Novel Drug Delivery by N.K. Jain, CBS Publishers & Distributors,
New Delhi, First edition 1997 (reprint in 2001).
• Novel Drug Delivery Systems by Y W. Chien, 2nd edition, revised and expanded, Marcel
Dekker, Inc., New York.
Evaluation Scheme Bachelor of Pharmacy I, II, III & IV Year syllabus 2019-2020 Page 114
