SARDAR PATEL UNIVERSITY
VallabhVidyanagar, Gujarat
(Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25))
Syllabus with effect from the Academic Year 2017-2018
M.Pharm (Pharmaceutical Quality Assurance)
Semester - I
Modern Pharmaceutical
Course Code MQA101T Title of the Course
Analytical Techniques
Total Credits
4 Hours per Week 4
of the Course
Main Focus of Employability Skill Development Entrepreneurship
the Corse
outcomes ü √
Course 1. The course is designed to impart the knowledge in the field of Pharmaceutical
Objectives: Analysis.
2. The various modern analytical techniques like UV-Visible, IR, NMR, Maas are
taught to enable students to understand and apply principles involved in the
determination of pharmaceuticals.
3. Chromatographic methods and other important topics are taught to enable the
students to understand and apply the principles involved in the determination of
pharmaceuticals
Course Content
Unit Description Hrs
UV-Visible spectroscopy: Introduction, Theory, Laws, Instrumentation associated
I with UV-Visible spectroscopy, Choice of solvents and solvent effect and 10
Applications of UV- Visiblespectroscopy.
IR spectroscopy: Theory, Modes of Molecular vibrations, Sample handling,
Instrumentation of Dispersive and Fourier - Transform IR Spectrometer, Factors
affecting vibrational frequencies and Applications of IRspectroscopy
Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence,
Quenchers, Instrumentation and Applications of fluorescencespectrophotometer.
Flame emission spectroscopy and Atomic absorption spectroscopy: Principle,
Instrumentation, Interferences and Applications.
NMR spectroscopy: Quantum numbers and their role in NMR, Principle,
II Instrumentation, Solvent requirement in NMR, Relaxation process, NMR signals 10
in various compounds, Chemical shift, Factors influencing chemical shift, Spin-
Spin coupling, Coupling constant, Nuclear magnetic double resonance, Brief
outline of principles of FT-NMR and 13C NMR. Applications of
NMRspectroscopy.
Mass Spectroscopy: Principle, Theory, Instrumentation of Mass Spectroscopy,
III 10
Different types of ionization like electron impact, chemical, field, FAB and
1
SARDAR PATEL UNIVERSITY
VallabhVidyanagar, Gujarat
(Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25))
Syllabus with effect from the Academic Year 2017-2018
MALDI, APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass
fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of
Mass spectroscopy
Chromatography: Principle, apparatus, instrumentation, chromatographic
IV 10
parameters, factors affecting resolution, isolation of drug from excipients, data
interpretation and applications of the following:
· Thin Layer chromatography
· High Performance Thin LayerChromatography
· Ion exchangechromatography
· Columnchromatography
· Gas chromatography
· High Performance Liquidchromatography
· Ultra High Performance Liquidchromatography
· Affinitychromatography
· Gel Chromatography
Electrophoresis: Principle, Instrumentation, Working conditions, factors affecting
V 10
separation and applications of the following:
a) Paper electrophoresis
b) Gel electrophoresis
c) Capillary electrophoresis
d) Zone electrophoresis
e) Moving boundary electrophoresis
f) Iso electricfocusing
X ray Crystallography: Production of X rays, Different X ray diffraction
methods, Bragg‘s law, Rotating crystal technique, X ray powder technique, Types
of crystals and applications of X- ray diffraction.
Potentiometry: Principle, working, Ion selective Electrodes and Application
VI 10
ofpotentiometry.
Thermal Techniques: Principle, thermal transitions and Instrumentation (Heat
flux and power-compensation and designs), Modulated DSC, Hyper DSC,
experimental parameters (sample preparation, experimental conditions, calibration,
heating and cooling rates, resolution, source of errors) and their influence,
advantage and disadvantages, pharmaceutical applications. Differential Thermal
Analysis (DTA): Principle, instrumentation
and advantage and disadvantages, pharmaceutical applications, derivative
differential thermal analysis (DDTA). TGA: Principle, instrumentation, factors
affecting results, advantage and disadvantages, pharmaceutical applications.
Teaching- 1. Students will be oriented about the course content in the first session of the
Learning course
Methodology 2. Class-room teaching will be based on interactive sessions using chalk and
board teaching method as well as teaching aids such as Powepoint
presentations, audio-visual presentations.
2
SARDAR PATEL UNIVERSITY
VallabhVidyanagar, Gujarat
(Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25))
Syllabus with effect from the Academic Year 2017-2018
3. Virtual teaching and examination will also utilized for the learners of the
course.
4. Seminar/assignments on advanced, recent and useful topics related to the
course will be an integral part of course teaching and learning methodology.
5. Quiz and Q & A sessions for the topics covered will be conducted at regular
interval.
6. Internal theory test will be conducted as a part of regular curriculum.
7. Attendance will be marked for each session as a part of overall evaluation.
8. Teaching will be facilitated by reading material, discussion forum, task-based
learning and case discussions.
Evaluation Pattern
Sr. Details of Evaluation Marks
No
1. Continuous Mode 10
2. Sessional Examination 15
3. End Semester Examination 75
CourseOutcomes:
Upon completion of the subject student shall be able to
1. Theorize the fundamentals of modern analytical techniques for the analysis of pharmaceuticals
2. Optimize the experimental conditions of modern pharmaceutical analytical instruments
3. Analyze or infer the results based on analytical data or observations recorded
4. Apply modern pharmaceutical analytical techniques for the analysis of pharmaceuticals
Suggested References:
Sr. No References
Spectrometric Identification of Organic compounds - Robert M Silverstein, Sixth edition,
1
John Wiley & Sons,2004
Principles of Instrumental Analysis - Doglas A Skoog, F. James Holler, Timothy A.
2
Nieman, 5th edition, Eastern press, Bangalore,1998.
3 Instrumental methods of analysis – Willards, 7th edition, CBSpublishers
Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th edition,
4
CBS Publishers, New Delhi,1997
5 Organic Spectroscopy - William Kemp, 3rd edition, ELBS,1991
Quantitative Analysis of Drugs in Pharmaceutical formulation - P D Sethi, 3rd
6
Edition, CBS Publishers, New Delhi,1997.
3
SARDAR PATEL UNIVERSITY
VallabhVidyanagar, Gujarat
(Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25))
Syllabus with effect from the Academic Year 2017-2018
Pharmaceutical Analysis - Modern Methods – Part B - J W Munson, Vol 11, Marcel.
7
DekkerSeries
8 Spectroscopy of Organic Compounds, 2ndedn., P.S/Kalsi, Wiley estern Ltd., Delhi
Textbook of Pharmaceutical Analysis, KA.Connors, 3rd Edition, John Wiley &
9
Sons,1982
10 Textbook of Pharmaceutical Analysis, KA.Connors, 3rd Edition, John Wiley & Sons,1982
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