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 Foamy Virus Case Study
Foamy virus (FV) containing a novel 36 bp insulator in the long terminal repeats region, induced
extremely low immortality in mouse hematopoietic stem cells. CRISPR/Cas9 mediated site specific
proviral sequence in LMO2 proto oncogene caused its high expression after removal of insulator
bound CCCTC factor of viral backbone while significantly decreased LMO2 expression found with
incorporated insulator.(Goodman et al., 2017)

Increased lengths of dystrophin open reading frame inserted foamy virus vector is a larger clinically
relevant packaging vector for transducing human muscle derived cells. Quantification of packaged
vector RNA and integrated DNA evidenced the reduced concentration titres up to 100 106 fold
transducing units per ml ... Show more content on Helpwriting.net ...
Expression of MxB in IFN β treated THP 1 and CRISPR/CAS THP 1 Knockout MxB cells showed
the same extend of restriction. MxB protein of IFN β targeting HIV had no impact on foamy
virus.(Bahr et al., 2016)

Mapping of retroviral vector integration sites (RIS) for evaluating novel gene therapy vectors to
track clonal contribution is not suggestible. Modified genomic sequencing PCR (MGS PCR)
protocol using Illumina MiSeq paired end sequencing platform for lentiviral, gamma retroviral and
foamy virus vector integrations is very efficient for localization of RIS.(Rae et al., 2015)

Human CD34(+) cord blood cells transduced with FV vector encoding methylguanine
methyltransferase (MGMT)P140K transgene, transplanted into immunodeficient NOD/SCID
IL2Rγ(null) mice. Polyclonal repopulation with no clonal dominance was found though using strong
internal spleen focus forming virus promoter known to be genotoxic.(Olszko et al., 2015)

Transportin 3 (TNPO3) has great role in replication of FVs. Viral integrase TNPO3 interaction is
important for nuclear import of FV pre integration complex. FV infection was reduced 2 fold by
knockdown (KD) of TNPO3.(Ali et al., 2015)

Authors constructed a novel recombinant PFV vector plasmids expressing three short hairpin RNAs
(shRNAs) targeting the conserved regions of the HIV 1NL4 3 5 long terminal repeat (LTR). These
vectors resulted a significant inhibition of HIV 1 replication without cytotoxicity, where shRNA3
was the most effective.(Cheng
 Essay on Public Service Broadcasting Within The Plural...
Introduction
British Broadcasting Corporation [BBC] has played its role as a Public Service Broadcasting
[PSB] in England since 1926. It was set up on Baron Reith s hands as an educative media building
public s character by establishing and socializing cultural value to the society via its programs.
Hitherto, BBC s work focuses on the cultural connection among the society and plays a role as its
guardian rather than pays attention to the diversity among them (Curran Seaton, 2003). However,
some critiques about its existence started raising in the end of 1980s as the appearance of the Sky
Media commercial satellite television company which gave larger opportunity for public to choose
what they want to see. Recently, one of the most ... Show more content on Helpwriting.net ...
Therefore, its operation focuses more on fostering the civilization and democracy rather than to gain
revenue. Besides, it also must be willing for a limited autonomy in its operation since the government
has the authority to control its working mechanism.
The core objection of this form of broadcasting is to serve public by providing quality information
and facilitating public discourse to enforce democracy and advance civilization. In term of
quality information, PSB plays a role as socio cultural agent whose duty is to perpetuate the high
culture and raise public awareness as an united national entity. Furthermore, in its role as a
discursive arena, PSB exists as a practice of Habermas idea of public sphere where public as a
political community exercise their freedom of assembly and freedom of information to solve their
social problem as well as to reach an agreement about the common good which is expected to affect
the policy in a society (Hauser, 1998).
To guarantee that this democratic function works well, PSB must guarantee that its operation is
impartially detached from either state or market interest. In this case, BBC is a good example.
Since it s mobilization from private company to public corporation, BBC operates as a form of
quangos (Butsch, 2007) which work as an independent organisation playing a governmental role.
Since 1946, its operational fund comes from the
 To Build A Fire By Stephen Crane, And Edith Wharton
When you find yourself lost in the woods, it would be a good idea to remember all those episodes
of Bear Grylls you watched while eating microwaved popcorn. Another idea is to not over think
your situation. Yes, you are lost in the woods, but it is no time to freak out and drink your own
pee. We learn in stories like To Build a Fire by Jack London, if a man thinks he is too big for his
britches, he will die in a frozen tundra. This theme of Man versus Natureis a central point of
Naturalistic and somewhat Realistic literature. These terms, although distinctly different in relation
of time period and works, both discuss the laws behind the forces that govern human lives, and seeks
to add an appeal tour our hidden instinctual behaviors.
Realism ... Show more content on Helpwriting.net ...
Jack London, Stephen Crane, and Edith Wharton are all considered great Naturalist writers. The
interesting thing about these writers is that while they write about similar topics surrounding the
awareness of Man versus many different forces, they all lead noticeably diverse lives. One was a
poor writer who traveled the continent while bracing the very same elements he wrote about, one
was a journalist who obtained recognition for works in his early academic years, and another was a
wealthy, divorced author who impacted the literary society of today. The primary aspects of
Naturalism include humans being in a struggle with forces beyond their control, attention to the
setting, and a unifying objective. Charles Darwin, although not alive during the Naturalistic
writing period, was a primary influence with his contribution of the concept of survival of the
fittest. To Build a Fire by Jack London is a great case of that notion. The main character of this
work has the thought process of All a man must do was to keep his head, and he was all right ,
and it does not seem to work out well for him (London). He is traveling toward his camp, and his
only other companion is a wolf dog. In this story, we have an omnipresent narrator, so we are able
to understand what the man and the dog are thinking. We see the man facing a multitude of forces.
Man versus nature, man versus instinct, man versus ego, all go against the man and provide
conflict. On the other side, the dog only faces the opposition of loyalty. Throughout the journey, the
dog always, knew enough to do this , whether that was to immediately lick of ice accumulated on
its feet, or to find fire or dig a hole to hide from the wind, or leave the dead guy behind (London).
The dog had instincts that the man did not, and when the man tried
 Language In Human Communication
Language is a powerful tool that is integral in human communication. The human brain is hard wired
to be adept at understanding social cues and situations that change the way we choose to
communicate. These internal and external factors play into one another and ultimately determine
how we choose to correspond with others to achieve our ultimate goal relaying a message.
Although in many cases it is frowned upon to rely on slang to convey ideas, in some situations,
slang can be a reliable resource. By using his or her discretion and analyzing the situation, the
speaker must determine whether or not this dangerous, risk reward weapon should be used.
Internal factors will greatly contribute to the path a person takes in corresponding with others. The
way a person feels about his or her own subject matter, determines what attitude the message will
carry. This is known as tone. A speaker who is speaking on something he or she disagrees with
might possess an antagonistic tone, while someone who is passionate about their topic may be
upbeat. The tone of a message arbitrates what words will be used. This is called the style. Style is
the culmination of word choice, idea arrangement, and the overall message portrayed by the
grouping of words. For example, the antagonistic speakers, may use a great deal of dysphemistic
language and arrange their ideas in a way that will cause their audience to view the subject of their
speech in a negative light. On the other hand, the passionate
 Personally Identifiable Information
Personally Identifiable Information What is it?
Personally Identifiable Information, or (PII), is any data that could possibly identify a unique
individual. Any information that can be used to identify one person from another can then be used to
cross reference with another source to re identify the unnamed individual can be considered PII.
PII is divided into two categories; Sensitive, and Non Sensitive.
According to Northeastern University, (2015) Sensitive PII is defined as: Information that if lost,
compromised, or disclosed could result in serious harm, embarrassment, inconvenience, or unfairness
to an individual . In general terms it is any information that could be used by criminals to conduct
identity theft, blackmail, stalking,
 Safety Lab Experience
When i was 12years old i was going to sience we were doing are safety lab so we were going to all
the stuff she show us the fire ectercure and it went off and hit the table then went in my eye i
think i was sharked that it went off i jumped up and run back and forth i was freaking out then my
teacher got me and ran me to the eye washer and i walk back and was trying to help clean the
mess but my eye buren bad so i went to the clinic they wach my eye out and told me to take off my
shirt and she gave me one she call my dad to let me talk to him my eye was all red. I think my
teacher thoughtthat the pin was till i the think when she thought it was was saved to pick it up and
pick it up and it went off i know she feel so so bad.She was saying
 Methyl Red And Vofes-Proskauer
After finish with SIM test, we jumped to MR VP Test which is a combination medium used for
both Methyl Red (MR) and Vofes Proskauer (VP). It s a solution containing peptone, glucose, and
phosphate buffer. MR Test is to detect organisms capable of performing a mixed acid fermentation,
and VP Test was designed for organisms that are able to ferment glucose, then convert their acid
products to acetoin and 2,3 butanediol. My result came out to be MR positive which was red color
and VP negative due to no color changed. The next test I had to do was Phenol Red Broth Test to
determine their fermentation characteristics. I only did Lactose and Sucrose. The test was done by
adding carbohydrateand use phenol red as pH indicator. Acid production from fermentation of the
carbohydrate will lower the pH below the neutral rang and turn the medium yellow, and Durham
tube is added to indicate if there was any gas production.
We continued to perform Decarboxylase Test which were Lysine and Ornithine to indicate if amino
acid was presented. If the organism is a glucose fermenter but does not produce decarboxylase the
medium will turn yellow and if the organism does not ferment glucose, the medium will remain
same color. In this case, purple stands for positive and others stands for negative. ... Show more
content on Helpwriting.net ...
One of the last test I needed to get done was Citrate Test which is used to determine the ability of
an organism to use citrate as its sole source of carbon. The result came out to be blue which was
citrate positive mean that citrate is utilized. Finally, the last one was Phenylalanine Deaminase
which can be identified by removing the amine group NH2 from the amino acid phenylalanine.
Ferric chloride (FeCl3) is used as reagent added to the medium after incubation. The result will turn
to dark green immediately indicates the presence of phenylpyruvic acid and yellow is
 Irony In The Screwtape Letters
A New Perspective Above all, do not attempt to use science (I mean, the real sciences) as a defense
against Christianity. They will positively encourage him to think about realities he can t touch and
see. Screwtape wrote this in the book by C.S Lewis called The Screwtape Letters. In The Screwtape
Letters Screwtape writes to his nephew, Wormwood, and instructs him on the proper ways to tempt
their patient . Unfortunately, in the end C.S Lewis implies that Screwtape committed cannibalism
and consumed his nephew due to the unfortunate (for them) results of Wormwood s attempted
tempting. In this satirical book, C.S Lewis shows how the devil attempts to tempt us to consume us
in Hell. C.S Lewis uses ironyand sarcasmto warn us of the dangers... Show more content on
Helpwriting.net ...
Showing us the ways the devil may attempt to tempt us through irony and sarcasm, C.S Lewis
wrote this book his hopes that one might read it and change their lifestyle. Although Wormwood
failed to convert the patient, many other people, Christians and non Christians alike, will enjoy the
irony and sarcasm C.S Lewis put in his fantastic book. This book really stretches your brain muscles
as you ponder many aspects placed in the book and look at the war between Heaven and Hell in a
new way and new
 Physical Therapy Is The Treatment
Physical Therapy is the treatment a person needs to assist in problems with the body. Physical
Therapy is a broad medical field that specializes in providing help to heal a person. Physical
therapy is the type of treatment a person may need when there are injuries to the body, or when
physical harm has occurred. Physical Therapyis a medical field that covers the mobility of the body.
Physical Therapyis involved with many parts of the body. It is the treatment a patient seeks when
assistance is needed to recover from an injury or illness that has affected the body.
When it is hard for a person to move around due to diseases to the body they should seek into
physical therapy for their body. Physical therapy helps people of all ages who ... Show more content
on Helpwriting.net ...
Also, they provided ways that will put less pressure on the body part that are not as strong as the
others.
A doctor may suggest physical therapy for some people with injuries. They may also suggest a
person consult with a physical therapist that has long term health problems such as arthritis or
any other disease. Physical therapy usually is done as one and is all that is needed. There may be
people who need surgery and then physical therapy afterwards. Or vice versa. Physical therapy
can help you live more easily these diseases that have affected the movement of your body. This
can range from spinal problems and Parkinson s disease. A physical therapist will work to set goals
to make doing daily tasks easier for you.
Physical therapy is a field that has many areas to cover. These areas are what a person may need
to heal any injuries or illness. The areas range from studies of the muscles to the bones. Physical
therapy also covers the areas of the skin. The skin is an odd area but can be used to detect a
problem. The skin can show a physical therapist where an injury may have occurred. The skin can
become red, blue, or swollen. This will alert the physical therapist of the area that needs care. The
muscles are parts of the body that need special attention. The muscles allow the body to move and
has a blood flow.
Physical therapy can help you recover from an injury. It can also help your body to be prepared for
an injury in the future. Physical therapy can
 Summary Of Emerson s The Age Of Innocence
A character who heeds Emerson s call to act on and obey no law less than the internal law pursues
individualistic ambitions, relying on personal beliefs and forming a life unburdened by customs or
social norms. Ellen Olenska heeds Emerson s call by physically and mentally resisting the
domineering advances of New York s aristocratic socialites, escaping the bonds that so securely
bind individual liberty and expression, and accepting a life that should be so bold as to venture past
the oppression of ballrooms and the shackles of conformity.

Ellen Olenska heeds Emerson s call by evading the jaws that so mercilessly snare personal
autonomy and individuality, daring to be so brave as to don outlandish attire and journey beyond the
coercion of ballrooms and the chains of convention. In The Age of Innocence, the Josephine look, is
depicted as [a] headdress... [which] [was] carried out in the cut of the dark blue velvet gown [that]
rather theatrically caught up under [the] bosom by a girdle with a large old fashioned clasp was a
style named after Josephine (1793 1814), the empress of Napoleon Boneparte (Wharton 7). In other
words, the Josephine Look was an elegant look that comprised of ornamental headbands and gowns
that were fastened under the bosom and donned over a sheer slip; it juxtaposed dramatically with the
lace covered décolletés and the prominent bodices that distinguished the dresses donned by stylish
American women in the 1870s. This deserves attention
 Essay about The Heroic Nora Helmer in Henrik Ibsen s A...
The Heroic Nora Helmer in Henrik Ibsen s A Doll s House What does it mean to be a hero?
According to Webster, a hero is someone of great strength [and] courage who is admired for his
or her courage and nobility. 1 Stretching this definition a bit further, I would argue that a hero is
someone who uses this strength, courage, and nobility to help or save others. Nora Helmer, in
Henrik Ibsen s A Doll s House, leaves her husband and family at the end of the play a move that can
be viewed as either very selfish or very heroic. Because Nora uses her strength and courage in effort
to save others and herself from the false doll s house life they are living in, her final act of leaving
home is truly heroic. Nora saves... Show more content on Helpwriting.net ...
This can be seen when she calls her youngest child her sweet little baby doll, and later when she
reassures her children that the doggies wouldn t bite [her] pretty little dollies (22).2 Nora seems
to be overlooking the fact that these little, doll like figures are real people that she is responsible
for. In this same scene, Nora plays hide and seek, laughing and shrieking, with her kids (23). As
the argument that Nora is being selfish states, she is her children s playmate. Yet, shouldn t a
mother be more than just a fellow playmate? A mother has a responsibility to teach and train her
children, not just play with them. This scene is the only time we see direct interaction between
Nora and her kids; it suggests that being a playmate or else using them as her play toys, are two
of the only ways Nora knows how to be a mother. Nora herself admits to Torvald, in their final
confrontation, that the children have been my dolls who thought it was fun when I went and
played with them (81). Later in the play, after this first scene with her children, when Nora is
talking to Anne Marie, we can see that she is making efforts to ensure that her children will be
cared for if even if she is not there to raise them. Anne Marie is the only mother Nora has ever had
and, as Nora says, she was a good mother to [Nora] when [she] was little (36). Nora goes on to say,
And if my little ones only had
 Charles I Outline
Mclntosh, Neil. Charles I (1600 1649). BBC.co.uk. United Kingdom. 2014. Web. 1 Dec. 2015.
Charles I was the king of England, Scotland and Ireland. His issues with parliament started the
civil war and caused his death. Charles I was born in Fife on November 19, 1600. He is the second
son of James VI of Scotland and Anne of Denmark. In 1603, Charles father, James, became king of
England and Ireland because Elizabeth had expired. Charles brother, Henry was the original heir of
the throne, but he passed away in 1612. Then Charles I became king in 1625. He then married
Henrietta Maria of France and had five kids with her. In the beginning of his reign, Charles had an
unpopular relationship with the duke of Buckingham, who used his power against ... Show more
content on Helpwriting.net ...
Charles had an unhealthy childhood. He was very weak as a boy but he grew up to be an
outstanding horsemen and a strong controlling king, which is what caused issues with parliament.
He inherited his father s financial problems, who was also unfriendly with parliament. Ongoing
problems with parliament led to his execution. Charles s wife s religion enraged the people of
England. Her friends that were also Catholic stood up for her in the courts. Charles s wife put her
and her friend s wants ahead of the realm. After being captured, Charles unleashed forces. This plan
of his majorly backfired, because he was assassinated right after. It was an obvious win of the
parliament supporters considering they outnumbered monarchy forces by far.
This source was accommodating, because it helped me learn about Charles s personality. His marks
on history are obvious, but it is hard identifying him as a personality. This source enabled me to
understand the kind of person king Charles was. Which will be useful for the presentation. It is
important to know a person s personality before making judgements.
Kiryanova, Elena. Images of Kingship. Charles I, Accession Sermons, and Theory of Divine Right.
100 339 (2015). Biography Reference Bank. Web. 4 Dec.
 Ethical Relativism And Moral Relativism
In this paper, I will argue against moral relativism as the correct way to judge human morality.
There are several issues with moral relativism, and I will focus on why moral relativism s argument
is false and also consider the implications of accepting relativism. I will propose an alternative
framework for making moral judgments about right and wrong and consider relativist objections.
Philosophers often vary in their usage of moral relativism, so, for the purpose of this paper, I will be
considering moral relativism to be the belief that moral judgments are right and wrong only relative
to the framework of a community. It follows from this that relativists also believe no moral
standpoint can be proved to be objectively superior to another.
Let us consider the argument that moral relativism proposes. Since a relativist believes that
moral absolutes do not exist, it follows that he must believe that all moral claims are only
preferences. For example, a relativist would say that the claim murder is wrong is true only if
and only if the speaker believes it is true. So, if I say Killing is wrong , it is morally true if I
actually believe what I say. It follows from this that, according to relativism, moral disagreements are
disagreements about preferences (as opposed to facts). This is because, as mentioned, relativism
believes morality to be preferences, so disagreements of morality must be disagreements over
preferences. Since this claim follows from the main argument,