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Introduction To The Microscope: Care Parts Focusing

The document provides instructions on how to use a compound microscope by explaining its main parts including the eyepiece, body tube, revolving nosepiece, objective lens, stage, stage clips, coarse and fine focus knobs, base, and light source. It describes how to properly carry, store, clean and focus the microscope. The stages of use involve placing the slide on the stage, adjusting the nosepiece to select low or high power objectives, and using the coarse and fine focus knobs to bring the specimen into view.

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71 views28 pages

Introduction To The Microscope: Care Parts Focusing

The document provides instructions on how to use a compound microscope by explaining its main parts including the eyepiece, body tube, revolving nosepiece, objective lens, stage, stage clips, coarse and fine focus knobs, base, and light source. It describes how to properly carry, store, clean and focus the microscope. The stages of use involve placing the slide on the stage, adjusting the nosepiece to select low or high power objectives, and using the coarse and fine focus knobs to bring the specimen into view.

Uploaded by

its jycdlcrz
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PPT, PDF, TXT or read online on Scribd
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Introduction to the

Microscope
Care
Parts
Focusing
• Always carry with 2 hands
• Only use lens paper for cleaning
• Do not force knobs
• Always store covered
• Keep objects clear of desk and cords
Parts of a Compound
Microscope

c om p ou n d
m i c r os c op e
Eyepiece
Body Tube

Revolving Nosepiece
Arm
Objective Lens

Stage
Stage
Clips Coarse Focus
Diaphragm
Fine Focus
Light

Base
Compound Microscope

• A microscope is a
very powerful
magnifying glass

• A microscope
helps you see
things like cells up
close
Eyepiece

• View the specimen


through the
eyepiece
Stage Clips & Objectives
• Stage clips hold the
slide in place
• Low power objective
is used to focus the
microscope (short &
fat)
• High power objective
is used to view
details of a specimen
Coarse Adjustment, Fine
Adjustment, & Base
• Coarse adjustment
focuses adjustment
• Fine adjustment fine
tunes & gives detailed
focus(usually smaller
than coarse
adjustment knob)
• Base is where the
microscope rests
Stage
• Stage is part
where the slide
rests
• Mirror (or light
source) directs
light upwards onto
the slide.
Diaphragm

• Diaphragm allows
light in
Nosepiece

• Nosepiece is the
rotating device
that holds the
objectives (lenses)
Arm

• Arm is the part


where you carry
the microscope
Can you name the parts of a
compound microscope?
Answers
• base
• mirror (light source)
• diaphragm
• stage
• stage clips
• low power objective lens
• high power objective lens
• nosepiece
• arm
• fine focus knob
• body tube
• coarse focus knob
• eyepiece
Types of Microscopes
Light microscope
• (for small objects -
similar to the way
binoculars magnify
objects far away.)
The Compound Light
Microscope

http://www.cas.muohio.edu/~mbi-ws/microscopes/compoundscope.html
• May have many lenses
that magnify the object
• Eyepiece = 10x
magnification
• Objective = 10x (low) or
40X (high)
•Total magnification=
•eyepiece X objective
•ex: 10x X 40x =
400x total
magnification
Compound Light Microscope
• Powered by Light & Lenses
a) Powers of Magnification
• Ocular Lens= _________
• Low Power Objective= _______
• Medium Power Objective=
_______
• High Power Objective= _______
• Calculating Total
Magnification= ____________
b) Resolution
c) Parts
Stereomicroscope
• To look at large things that light
cannot pass through (one eyepiece
for each eye)
Stereomicroscopes

http://scientificsonline.com/product.asp_Q_pn_E_3119600

http://www.martinmicroscope.com/MicroscopePages/Stereomicroscopes.htm
Stereomicroscopes
•3 - D image
•can only magnify 10x
to 600x
Electron Microscope
• can magnify more
than 500,000x
House Fly

• http://www.pbrc.hawaii.edu/bemf/microangela/hfly.htm
• Place the Slide on the
Microscope
• Use Stage Clips
• Click Nosepiece to the lowest
(shortest) setting
• Look into the Eyepiece
• Use the Coarse Focus
• Follow steps to focus using low power
• Click the nosepiece to the longest objective
• Do NOT use the Coarse Focusing Knob
• Use the Fine Focus Knob to bring the slide
into focus

What can you find on your slide?

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