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Compound Microscope Guide

The document summarizes the key parts and proper use of a compound microscope. It identifies the main components, including the ocular lens, revolving nosepiece, objective lens, stage, condenser, and illuminator. It explains that total magnification is determined by multiplying the power of the ocular lens by the power of the objective lens. The document also provides instructions for proper handling of the microscope, such as carrying it upright with two hands and rotating the nosepiece by its outer disk.

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Kyle Labor
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142 views10 pages

Compound Microscope Guide

The document summarizes the key parts and proper use of a compound microscope. It identifies the main components, including the ocular lens, revolving nosepiece, objective lens, stage, condenser, and illuminator. It explains that total magnification is determined by multiplying the power of the ocular lens by the power of the objective lens. The document also provides instructions for proper handling of the microscope, such as carrying it upright with two hands and rotating the nosepiece by its outer disk.

Uploaded by

Kyle Labor
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
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THE COMPOUND

MICROSCOPE
By: Rojen G. Mana-ay, RMT
A microscope __________
MAGNIFIESthe
image of a specimen.
Occular Lens

Diopter
Head
Adjustment

Revolving Nosepiece
Arms
Objective Lens
Stage Clips
Stage
Aperture
Coarse Adjustment Knob
Diaphragm
Condenser Fine Adjustment Knob

Illuminator Stage Controls


(Light Source)
Base

Brightness Adjustment

Light Switch
Light intensity may also be adjusted by
adjusting the diaphragm just below the stage.

X
A condenser adjusts the light
intensity of a microscope.

STAGE
A platform below the objective.
X
The COARSE
fine focusfocus
knobknob
changes
changes
the distance
the
greatly while
distance GREATLY
coarsewhile
focusFINE
knob
focus
changes
knob
the distance
changes the distance
very little.VERY LITTLE.

It is a lens that concentrates light, may be


CONDENSER
found between the lamp and the stage.
PARTS OF A COMPOUND MICROSCOPE
Total magnification
– determined by multiplying the
If you select a 100X objective to use
power of the ocular by the
with a 5X ocular, the total
power of the objective.
magnification is
– For example:

• Power of LPO = 10x500X


• Power of ocular = 10x

• Total magnification: 100x


X
SCANNER – 4X
40x objective
LPO – 10X
HPO – 40X
requires the use of
OIO – 100X
oil when viewing a
specimen.
PROPER HANDLING AND CORRECT MANIPULATION OF THE MICROSCOPE.

X
Always carry the scope with two hands: one under the base
and the other grasping the arm. Carry in an upright position.
PROPER HANDLING AND CORRECT MANIPULATION OF THE MICROSCOPE.

keep both eyes closed


1. X
To avoid eyestrain and a possible headache, always
OPEN when
when viewing
viewing aa specimen.
specimen.
Always carry the microscope with two hands: one under the base and the other
grasping the arm. Carry it in an upright position.
Always rotate the nosepiece by holding the outside
2. Carefully unwind the of
lamp
thecord. Avoid damaging
revolving the push
disk - never parts around
on the lens barrel.
which is coiled. Plug the cord into an outlet in a safe manner.

3. Make sure the stage and the objective are at their farthest distance apart and
Most scopes are parfocal which means that the image
that the
remains lowest when
focused power you
objective
changeis inobjectives.
position. Start each new observation

at low power.
X
Facial tissues or wipes can be used in
cleaning the lenses.

X
Always move the objective and stage toward each
other while looking through the ocular.

NEVER move the objective and stage toward each


other while looking through the ocular.

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