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An Outer Membrane Receptor of Neisseria meningitidis Involved in Zinc Acquisition with Vaccine Potential

Figure 1

Regulation of znuD gene expression.

Western blots of cell lysates using rabbit antiserum against ZnuD. (A) HB-1 grown in TSB (lane 1), RPMI (lane 2) and the znuD knockout strain grown in RPMI (lane 3). (B) HB-1 grown in RPMI supplemented with 0, 2, 4, 6 and 8% TSB (lanes 1–5, respectively). (C) HB-1 grown in RPMI supplemented with 0, 2, 5, and 10 µM FeCl3 (lanes 1–4, respectively). (D) HB-1 grown in RPMI supplemented with 0, 0.5 or 1 µM ZnSO4 (lanes 1–3, respectively). (E) HB-1 grown in RPMI supplemented with 0, 0.1, 0.5 or 1 µM TPEN (lanes 1–4, respectively). (F) HB-1 (lanes 1–3) and the zur mutant (lanes 4–6) grown in RPMI (lanes 1 and 4), RPMI with 0.6 µM ZnSO4 (lanes 2 and 5) or TSB (lanes 3 and 6).

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1000969.g001