The leucine-rich repeats in allelic barley MLA immune receptors define specificity towards sequence-unrelated powdery mildew avirulence effectors with a predicted common RNase-like fold
Fig 10
AVRA10 amino acid residues that are responsible for specific recognition correlate with residues that interact with the MLA10 receptor.
(A) N. benthamiana plants were transformed transiently with vectors containing cDNAs of Mla10-cLUC together with cDNAs of AVRa10-nLUC without signal peptide, chimera22-nLUC, chimera26-nLUC or chimera29-nLUC under the control of a 35S promoter. LUC activity was determined 40 h after A. tumefaciens-mediated transformation. The experiment was performed on at least four independent days with two to four replicates (independent set of plants) each day. Significant differences between samples were analyzed using non-parametric Kruskal-Wallis (KW) analysis followed by the Dunn’s test. Calculated KW p-value = 5.03e-05. Samples labeled with different letters differed significantly (p < 0.05) in the Dunn’s test. (B) Protein levels of AVRA10-nLUC, chimera22-nLUC, chimera26-nLUC and chimera29-nLUC. Proteins were separated on a 8% SDS_PAGE gel and a detected using anti-LUC western blotting (WB).